Comparative Genomic Analysis of Pseudoxanthomonas sp.X-1, a Bromoxynil Octanoate-Degrading Bacterium,and Its Related Type Strains
Comparative Genomic Analysis of Pseudoxanthomonas sp.X-1, a Bromoxynil Octanoate-Degrading Bacterium,and Its Related Type Strains
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辛酸溴苯腈降解菌假黄单胞菌 X-1 及其相关菌株的基因组比较分析
DOI：
10.1007/s00284-021-02735-y
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发表时间：
2022
影响因子：
2.6
通讯作者：
Xihui Xu
中科院分区：
文献类型：
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作者：
Zhepu Ruan;Weimiao Cao;Jingzhi Zhu;Bingang Yang;Ji;ong Jiang;Chen Chen;Xihui Xu
所描述的大多数假黄病菌来自水、植物或受污染的土壤。这是一株伪黄病菌。报道了从辛酸溴苯腈（BO）污染土壤中分离得到的X-1。菌株X-1能降解BO并产生溴苯醚。菌株X-1降解BO的最佳条件为BO初始浓度为0.1 mM，温度为30°C， pH为7，Mn2+浓度为1.0 mM。菌株X-1的细菌形态、生理生化特征与其他类型菌株存在差异。我们对菌株X-1的基因组进行了测序，并对X-1和其他假黄单胞菌进行了比较基因组分析，以探讨这些菌株之间差异的机制。菌株X-1的基因组编码4160个基因，其中蛋白编码基因4078个，RNA编码基因68个。具体而言，菌株X-1编码的酶属于778个酶委员会（Enzyme Commission， EC）编号，远多于其他相关菌株，其中62个是唯一的。在菌株X-1中检测到8个编码酯酶的基因，这导致了BO的降解能力。本研究为微生物修复除草剂BO污染的环境提供了菌株、酶和基因组资源。
MostPseudoxanthomonasspecies described have been derived from water, plants, or contaminated soils. Here, a strainPseudoxanthomonassp. X-1 isolated from bromoxynil octanoate (BO)-contaminated soil is presented. Strain X-1 could degrade BO and produce bromoxynil. The optimal conditions for degradation of BO by strain X-1 were an initial BO concentration of 0.1 mM, 30 °C, pH 7, and Mn2+concentration of 1.0 mM. The bacterial morphological, physiological, and biochemical characteristics of strain X-1 were described, which showed differences comparing with other related type strains. The genome of strain X-1 was sequenced, and a comparative genomic analysis of X-1 and otherPseudoxanthomonasspecies was conducted to explore the mechanisms underlying the differences among these strains. The genome of strain X-1 encodes 4160 genes, 4078 of which are protein-coding genes and 68 are RNA coding genes. Specifically, strain X-1 encodes enzymes belonging to 778 Enzyme Commission (EC) numbers, much more than those of other related strains, and 62 of them are unique. Eight genes coding esterase are detected in strain X-1 which leads to the ability of BO degradation. This study provides strain, enzyme, and genome resources for the microbial remediation of environments polluted by herbicide BO.