Effect of Probiotic strains on interleukin 8 production by HT29/19A cells
Effect of Probiotic strains on interleukin 8 production by HT29/19A cells
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DOI：
10.1111/j.1572-0241.2002.05693.x
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发表时间：
2002-05-01
影响因子：
9.8
通讯作者：
Campieri, M
中科院分区：
文献类型：
--
作者：
Lammers, KM;Helwig, U;Campieri, M
目的：研究益生菌在炎症性肠病维持治疗和预防膀胱炎中的作用。方法：评价临床研究中使用的益生菌(VSL#3、Escherichia Nissle 1917和LactobacillusGG)是否能够诱导上皮细胞产生趋化因子。HT29/19A与单株益生菌的细胞碎片和细胞提取液按103~109个菌落单位/毫升的剂量孵育32h，用ELISA检测培养上清液中IL-8的含量。结果：VSL#3和乳杆菌GG的乳杆菌和双歧杆菌不能诱导IL-8的产生，而大肠杆菌Nissle 1917的细胞碎片和细胞提取物均能诱导IL-8的产生，且呈剂量依赖关系。结论：在该培养模型中，革兰氏阳性益生菌不能诱导IL-8的产生，而非致病性革兰氏阴性杆菌Nissle 1917株则以剂量依赖的方式诱导IL-8的产生。这些结果表明，益生菌革兰氏阳性菌和大肠杆菌Nissle1917可能通过不同的作用机制对宿主产生有益的影响。
OBJECTIVES: Promising results from clinical studies on the effect of probiotics as maintenance therapy in inflammatory bowel disease and in the prevention of onset of pouchitis ask for studies to unravel the still poorly understood mechanism of action of probiotics.METHODS: To evaluate whether the probiotic bacteria that were used in the clinical studies (VSL#3, Escherichia coli Nissle 1917, and Lactobacillus GG) are able to induce chemokine production in epithelial cells, HT29/19A monolayers were incubated with cell debris and cell extract fractions of single strains of the probiotic bacteria in doses ranging from 103 to 109 colony-forming units/ml for 32 h. Supernatants were measured for interleukin 8 by ELISA.RESULTS: Lactobacilli and bifidobacteria strains from VSL#3 and Lactobacillus GG did not induce interleukin 8, whereas both cell debris and cell extracts from E. coli Nissle 1917 induced interleukin 8 production in a dose-dependent way. Cell extracts from streptococcal strains induced interleukin 8 when applied at high concentrations.CONCLUSIONS: Probiotic Gram-positive bacteria did not induce interleukin 8, whereas the nonpathogenic, Gram-negative E. coli Nissle 1917 strain induced interleukin 8 in a dose-dependent way in this culture model. These results suggest that probiotic Gram-positive bacteria and E. coli Nissle 1917 may exert their beneficial effects on the host by a different mechanism of action.