Follicular oocytes better support development in rabbit cloning than oviductal oocytes.
Follicular oocytes better support development in rabbit cloning than oviductal oocytes.
复制标题
卵泡卵母细胞比输卵管卵母细胞更好地支持兔子克隆的发育。
DOI：
10.1089/cell.2011.0030
复制
发表时间：
2011
影响因子：
1.6
通讯作者：
Du,Fuliang
中科院分区：
文献类型：
--
作者：
Sung,Li-Ying;Chen,Chien-Hong;Xu,Jie;Lin,Tzu-An;Su,Hwa-Yun;Chang,Wei-Fang;Liu,Chia-Chia;Sung,Yun-Shao;Cheng,WinstonTK;Zhang,Jifeng;Tian,XCindy;Ju,Jyh-Cherng;Chen,YEugene;Wu,Shinn-Chih;Du,Fuliang
本研究旨在探讨兔卵巢或输卵管卵母细胞对核移植胚胎发育潜能的影响。供体核来自成人皮肤成纤维细胞、卵丘细胞和胚胎卵裂球。注射hcg后10、11或12小时，将兔卵母细胞从输卵管（输卵管卵母细胞）中冲洗或从卵巢（卵泡卵母细胞）中吸出。收集到的大部分卵母细胞仍附着在卵巢上的排卵部位。我们发现卵泡卵母细胞与核供体细胞的融合率明显高于输卵管卵母细胞。卵泡组和输卵管组的卵裂率无显著差异，但卵泡组的桑葚胚和囊胚发育明显高于输卵管组。卵泡组使用卵裂球和积云核供体获得2个活克隆，输卵管组使用积云核供体获得1个活克隆。结果表明，卵泡卵母细胞克隆的兔胚胎比输卵管卵母细胞克隆的兔胚胎具有更好的发育能力。
This study was conducted to determine the effect of rabbit oocytes collected from ovaries or oviducts on the developmental potential of nuclear transplant embryos. Donor nuclei were obtained from adult skin fibroblasts, cumulus cells, and embryonic blastomeres. Rabbit oocytes were flushed from the oviducts (oviductal oocytes) or aspirated from the ovaries (follicular oocytes) of superovulated does at 10, 11, or 12 h post-hCG injection. The majority of collected oocytes were still attached to the sites of ovulation on the ovaries. We found that follicular oocytes had a significantly higher rate of fusion with nuclear donor cells than oviductal oocytes. There was no difference in the cleavage rate between follicular and oviductal groups, but morula and blastocyst development was significantly higher in the follicular group than in the oviductal group. Two live clones were produced in follicular group using blastomere and cumulus nuclear donors, whereas one live clone was produced in the oviductal group using a cumulus nuclear donor. These results demonstrate that cloned rabbit embryos derived from follicular oocytes have better developmental competence than those derived from oviductal oocytes.