In Vivo Imaging and Toxicity Assessments of Fluorescent Nanodiamonds in Caenorhabditis elegans
In Vivo Imaging and Toxicity Assessments of Fluorescent Nanodiamonds in Caenorhabditis elegans
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DOI：
10.1021/nl1021909
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发表时间：
2010-09-01
期刊：
影响因子：
10.8
通讯作者：
Chang, Huan-Cheng
中科院分区：
文献类型：
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作者：
Mohan, Nitin;Chen, Chao-Sheng;Chang, Huan-Cheng
纳米碳材料在生物技术和生物医学应用中具有巨大的前景。荧光纳米金刚石（FND）是最近加入纳米碳家族的新成员。在这里，我们报告了在秀丽隐杆线虫（C。elegans），并探索这种新型纳米材料与模式生物之间的纳米生物相互作用。将FND引入野生型C。通过用胶体FND溶液喂养它们或将FND悬浮液显微注射到蠕虫的性腺中，可以使线虫死亡。在进食时，裸露的FND留在肠腔中，而与生物分子（如葡聚糖和牛血清白蛋白）缀合的FND被吸收到肠细胞中。在显微注射时，FND分散在性腺中并递送到胚胎中，并最终进入下一代孵化的幼虫中。通过使用寿命和生殖潜力作为生理指标并使用报告基因测量应激反应进行的毒性评估表明，FND稳定无毒，不会对蠕虫造成任何可检测的应激。这种纳米材料的高亮度、优异的光稳定性和无毒性质使其能够连续几天对整个消化系统进行成像，并跟踪生物体的细胞和发育过程。
Nanoscale carbon materials hold great promise for biotechnological and biomedical applications. Fluorescent nanodiamond (FND) is a recent new addition to members of the nanocarbon family. Here, we report long-term in vivo imaging of FNDs in Caenorhabditis elegans (C. elegans) and explore the nano-biointeractions between this novel nanomaterial and the model organism. FNDs are introduced into wild-type C. elegans by either feeding them with colloidal FND solution or microinjecting FND suspension into the gonads of the worms. On feeding, bare FNDs stay in the intestinal lumen, while FNDs conjugated with biomolecules (such as dextran and bovine serum albumin) are absorbed into the intestinal cells. On microinjection, FNDs are dispersed in the gonad and delivered to the embryos and eventually into the hatched larvae in the next generation. The toxicity assessments, performed by employing longevity and reproductive potential as physiological indicators and measuring stress responses with use of reporter genes, show that FNDs are stable and nontoxic and do not cause any detectable stress to the worms. The high brightness, excellent photostability, and nontoxic nature of the nanomaterial have enabled continuous imaging of the whole digestive system and tracking of the cellular and developmental processes of the living organism for several days.