CIRCULATING EPSTEIN-BARR VIRUS-CARRYING B-CELLS IN ACUTE MALARIA
CIRCULATING EPSTEIN-BARR VIRUS-CARRYING B-CELLS IN ACUTE MALARIA
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DOI：
10.1016/0140-6736(91)90203-2
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发表时间：
1991-04-13
期刊：
影响因子：
168.9
通讯作者：
CRAWFORD, DH
中科院分区：
文献类型：
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作者：
LAM, KMC;SYED, N;CRAWFORD, DH
EB 病毒 (EBV) 感染和恶性疟原虫疟疾是地方性伯基特淋巴瘤病因学中的两个已知辅助因素。 为了评估这些因素之间的关系，使用有限稀释分析来评估急性疟疾期间和之后冈比亚儿童循环中携带 EBV 的 B 细胞数量。 急性疟疾患者和英国传染性单核细胞增多症患者的携带病毒细胞数量是恢复期疟疾患者和英国健康对照成年人的五倍。 阿昔洛韦（一种病毒 DNA 聚合酶抑制剂）可抑制急性疟疾样本有限稀释培养物中的自发生长。 因此，生长的机制是体内感染细胞释放病毒，导致共培养的正常 B 细胞感染并永生化。 研究结果证明，急性疟疾与循环中携带 EBV 的 B 细胞数量增加有关。 由于这种增加，此类细胞中发生细胞遗传学异常的可能性更大，从而导致伯基特淋巴瘤的演变。
Epstein-Barr virus (EBV) infection and Plasmodium falciparum malaria are two known cofactors in the aetiology of endemic Burkitt's lymphoma. To assess the relation between these factors, limiting dilution analysis was used to assess the number of EBV-carrying B cells in the circulation of Gambian children during and after acute malaria. Numbers of virus-carrying cells were five times higher in acute malaria patients and in UK patients with infectious mononucleosis than in convalescent malaria patients and in healthy control adults from the UK. Spontaneous outgrowth in limiting dilution cultures from acute malaria samples was inhibited by acyclovir, a viral DNA polymerase inhibitor. The mechanism of outgrowth, therefore, was virus release from the in-vivo infected cell, which led to infection and immortalisation of co-cultured normal B cells. The findings provide evidence that acute malaria is associated with an increase in the number of EBV-carrying B cells in the circulation. Because of this increase, there is a greater chance of a cytogenetic abnormality occurring in such a cell, with consequent evolution of Burkitt's lymphoma.