Role of catalase and oxyR in the viable but nonculturable state of Vibrio vulnificus
Role of catalase and oxyR in the viable but nonculturable state of Vibrio vulnificus
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DOI：
10.1016/j.femsec.2004.06.004
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发表时间：
2004-11-22
影响因子：
4.2
通讯作者：
Oliver, JD
中科院分区：
文献类型：
--
作者：
Kong, IS;Bates, TC;Oliver, JD
创伤弧菌已被证明在冬季难以从水或贝类中培养，这归因于活的但不可培养（VBNC）状态。由于发现活性氧参与低温诱导的创伤弧菌进入这种状态，我们产生了缺乏过氧化氢酶活性的oxyR突变体。由于固体培养基中存在H2O2，因此即使在环境温度下，该菌株也不能在固体培养基上培养。低温孵育的亲本导致过氧化氢酶活性的损失，使细胞H2O2敏感，并平行的可培养性的损失（进入VBNC状态）。因此，处于VBNC状态的创伤弧菌细胞可能对低原位温度表现出这种反应，并且只有当尝试实验室培养的人工条件时，细胞才由于冷诱导的过氧化氢酶活性丧失而不可培养。据我们所知，这是第一个研究提供直接证据的代谢基础的nonculturability和可行的，但nonculturable状态。(C)2004年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
Vibrio vulnificus has proven difficult to culture from water or shellfish during winter months, which is attributed to the viable but nonculturable (VBNC) state. Because reactive oxygen species were found to be involved in the low temperature-induced entrance of V. vulnificus into this state, we generated an oxyR mutant which lacks catalase activity. This strain is nonculturable on solid media even at ambient temperature, due to the presence of H2O2 in such media. Low temperature incubation of the parent resulted in loss of catalase activity, making the cells H2O2 sensitive, and paralleling the loss of culturability (entry into the VBNC state). Thus, cells of V. vulnificus in the VBNC state are likely exhibiting this response to low in situ temperature and only When the artificial condition of laboratory culture is attempted are the cells nonculturable due to cold-induced loss of catalase activity. To our knowledge, this is the first study providing direct evidence for the metabolic basis of nonculturability and the viable but nonculturable state. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.