DERMAL CONTACT WITH TOLUENE DIISOCYANATE (TDI) PRODUCES RESPIRATORY-TRACT HYPERSENSITIVITY IN GUINEA-PIGS
DERMAL CONTACT WITH TOLUENE DIISOCYANATE (TDI) PRODUCES RESPIRATORY-TRACT HYPERSENSITIVITY IN GUINEA-PIGS
复制标题
DOI：
10.1016/0041-008x(81)90426-9
复制
发表时间：
1981-01-01
影响因子：
3.8
通讯作者：
MAGRENI, CM
中科院分区：
文献类型：
--
作者：
KAROL, MH;HAUTH, BA;MAGRENI, CM
工业工人对TDI [甲苯二异氰酸酯]的肺超敏反应的发生是有据可查的。敏感性可能是由于在工作场所吸入TDI蒸气所致。这得到了基于重复吸入TDI的肺敏感性动物模型的支持（Karol等人）。工业工人经常因溢出物或飞溅物而通过皮肤接触暴露于TDI。使用豚鼠作为动物模型研究了皮肤暴露导致肺敏感性的可能性。将浓度范围为1-100%的TDI应用于豚鼠的背部部位。暴露后第7天，皮肤对TDI的敏感性（接触敏感性）明显。14天后，通过血清学分析和支气管激发试验额外评价动物的TDI敏感性。采用被动皮肤过敏反应、凝胶双扩散和放射性标记抗原结合（Farr）试验检测TDI抗体。抗体对TDI具有特异性，与TDI、二苯基甲烷二异氰酸酯（MDI）或二环己基甲烷二异氰酸酯（氢化MDI）的交叉反应性很小（如果有的话）。通过吸入0.005 ppm TDI或TDI-蛋白质结合物或对甲苯基异氰酸酯与蛋白质结合物气雾剂激发，可证明对TDI的呼吸道超敏反应。由于皮肤接触TDI而导致的呼吸道超敏反应的发展强调了谨慎的工作场所实践的重要性，并建议重新评估吸入和/或皮肤接触在引起工人肺部致敏中的作用。
The occurrence of pulmonary hypersensitivity to TDI [toluene diisocyanate] in industrial workers is well documented. Sensitivity probably results from inhalation of TDI vapors in the workplace. This was supported by an animal model for pulmonary sensitivity based on repeated inhalation of TDI (Karol et al.). Industrial workers are exposed to TDI through skin contact frequently as a result of spills or splashes. The possibility of dermal exposures resulting in pulmonary sensitivity was investigated using guinea pigs as an animal model. TDI in concentrations ranging from 1-100% was applied to dorsal sites on guinea pigs. Skin sensitivity (contact sensitivity) to TDI was apparent by the 7th day following exposure. After 14 days, animals were additionally evaluated for TDI sensitivity by serologic analysis and by bronchial provocation challenge. Antibodies to TDI were detected using passive cutaneous anaphylaxis, double diffusion in gel and radiolabeled antigen binding (Farr) assays. Antibodies were specific for TDI and showed little, if any, cross-reactivity with hexamethylene diisocyanate, diphenylmethane diisocyanate (MDI) or dicyclohexylmethane diisocyanate (hydrogenated MDI). Respiratory hypersensitivity to TDI could be demonstrated by inhalation challenge with 0.005 ppm TDI, or aerosols of either TDI-protein conjugates or conjugates of p-tolyl isocyanate with protein. The development of respiratory hypersensitivity as a result of dermal contact with TDI emphasizes the importance of careful workplace practices and suggests reevaluation of the role of inhalation and/or dermal contact in causing pulmonary sensitization of workers.