Digital counting of nucleic acid targets using solid-state nanopores
Digital counting of nucleic acid targets using solid-state nanopores
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DOI：
10.1039/d0nr03878d
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发表时间：
2020-09-14
期刊：
影响因子：
6.7
通讯作者：
Godin, Michel
中科院分区：
文献类型：
--
作者：
Beamish, Eric;Tabard-Cossa, Vincent;Godin, Michel
靶向用于疾病早期诊断的生物标志物的测定需要具有高度特异性和灵敏度的传感平台。在这项工作中，我们开发并表征了一种基于固态纳米孔的传感检测方法，用于检测具有易于定制的纳米结构DNA探针集的短核酸靶。我们探索了三种DNA纳米结构的电特征，以确定它们作为探针组在数字计数方案中的性能，以量化靶的浓度。利用这些探针，我们证明了在2重测定中同时特异性检测两种不同的DNA靶点，并分别检测与各种人类癌症相关的生物标志物microRNA-155。除了特异性靶点检测外，我们的方案还证明了定量至少六种不同microRNA浓度的能力。这些结果突出了固态纳米孔作为未来数字诊断技术的单分子计数器的潜力。
Assays targeting biomarkers for the early diagnosis of disease demand a sensing platform with a high degree of specificity and sensitivity. In this work, we developed and characterized a solid-state nanopore-based sensing assay for the detection of short nucleic acid targets with readily customizable nanostructured DNA probe sets. We explored the electrical signatures of three DNA nanostructures to determine their performance as probe sets in a digital counting scheme to quantify the concentration of targets. With these probes, we demonstrate the specific, simultaneous detection of two different DNA targets in a 2-plex assay, and separately that of microRNA-155, a biomarker linked to various human cancers. In addition to specific target detection, our scheme demonstrated the ability to quantify at least six different microRNA concentrations. These results highlight the potential for solid-state nanopores as single-molecule counters for future digital diagnostic technologies.