Ribbon modulates apical membrane during tube elongation through Crumbs and Moesin
Ribbon modulates apical membrane during tube elongation through Crumbs and Moesin
复制标题
DOI：
10.1016/j.ydbio.2008.05.541
复制
发表时间：
2008-08-01
影响因子：
2.7
通讯作者：
Andrew, Deborah J.
中科院分区：
文献类型：
--
作者：
Kerman, Bilal E.;Cheshire, Alan M.;Andrew, Deborah J.
虽然上皮管的形成和维持对于多细胞生物的生存能力是必不可少的，但我们对协调小管发生的分子和细胞事件的理解相对有限。在这里，我们重点关注Ribbon（一种含有核蛋白的新型BTB结构域）在果蝇唾液腺和气管这两个上皮管伸长中的活动。我们发现，丝带与Lola样，另一个BTB结构域含有蛋白质的强大的核定位的丝带，上调面包屑的表达和下调膜突蛋白活性。我们通过TEM对带状空唾液腺的超微结构分析揭示了顶端下囊泡的减少和微绒毛结构的增加，细胞变化与已知的顶端膜生成中的碎屑和膜突蛋白在顶端膜与顶端下细胞骨架的交联中的作用一致。此外，Rab11，一个小的GTdR与顶端膜的交付和重排，在带状突变唾液腺和气管的根尖下的本地化显着减少。这些发现表明Ribbon和Lola Like作为一种新的转录盒发挥作用，协调上皮细胞顶膜的分子变化以促进管的伸长。(c)2008年爱思唯尔公司All rights reserved.
Although the formation and maintenance of epithelial tubes are essential for the viability of multicellular organisms, our understanding of the molecular and cellular events Coordinating tubulogenesis is relatively limited. Here, we focus on the activities of Ribbon, a novel BTB-domain containing nuclear protein, in the elongation of two epithelial tubes: the Drosophila salivary gland and trachea. We show that Ribbon interacts with Lola Like, another BTB-domain containing protein required for robust nuclear localization of Ribbon, to upregulate crumbs expression and downregulate Moesin activity. Our ultrastructural analysis of ribbon null salivary glands by TEM reveals a diminished pool of subapical vesicles and an increase in microvillar structure, cellular changes consistent with the known role Of Crumbs in apical membrane generation and of Moesin in the cross-linking of the apical membrane to the subapical cytoskeleton. Furthermore, the subapical localization of Rab11, a small GTPase associated with apical membrane delivery and rearrangement, is significantly diminished in ribbon mutant salivary glands and tracheae. These findings suggest that Ribbon and Lola Like function as a novel transcriptional cassette Coordinating molecular changes at the apical membrane of epithelial cells to facilitate tube elongation. (c) 2008 Elsevier Inc. All rights reserved.