Studies on the mechanism of aluminum tolerance in pea (Pisum sativum L.) using aluminum-tolerant cultivar 'Alaska' and aluminum-sensitive cultivar 'Hyogo'
Studies on the mechanism of aluminum tolerance in pea (Pisum sativum L.) using aluminum-tolerant cultivar 'Alaska' and aluminum-sensitive cultivar 'Hyogo'
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DOI：
10.1080/00380768.2004.10408468
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发表时间：
2004-04-01
影响因子：
2
通讯作者：
Matsumoto, H
中科院分区：
文献类型：
--
作者：
Kobayashi, Y;Yamamoto, Y;Matsumoto, H
The mechanism of aluminum (Al) tolerance was investigated in an Al-tolerant cultivar (Alaska) and an Al-sensitive cultivar (Hyogo) of pea (Pisum sativum L.) seedlings. Al sensitivity was evaluated as the degree of root elongation inhibition in a calcium solution. Fifty percent inhibition of the elongation was observed after 24-h treatment with 5 muM Al in Alaska and with less than 2.5 muM Al in Hyogo. At 10 muM Al, both cultivars exhibited more than 70% inhibition. At 2.5 muM and 5 muM, Al triggered citrate secretion in both cultivars to the same extent, and the,levels of Al accumulation in root apices were similar in both cultivars, indicating that the difference in Al tolerance observed in these cultivars was not related to the exclusion of Al with organic acid. Al treatment enhanced the production of reactive oxygen species (ROS) specifically in the elongation zone in both cultivars. Al dose and time course experiments indicated the existence of a strong positive correlation between the root elongation inhibition and ROS production in both cultivars. At 10 muM, Al inhibited the respiration rate in the root apex by 20% in Alaska and 30% in Hyogo but not significantly at lower concentrations. In the absence of Al, the ATP content in the root apex was 2 times higher in Alaska than in Hyogo. With the increase in Al concentration, the ATP content in Alaska decreased and reached the same level in Hyogo at 5 muM Al, while the ATP content in Hyogo did not change. These findings suggest that Al-triggered ROS production is a key factor for the root elongation inhibition in both cultivars, and the possible involvement of high ATP content in Alaska in the protection mechanism against ROS production and root elongation inhibition under Al stress.
