Preserved ex vivo inflammatory status in decidual cells from women with preterm labor and subclinical intrauterine infection.
Preserved ex vivo inflammatory status in decidual cells from women with preterm labor and subclinical intrauterine infection.
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DOI：
10.1371/journal.pone.0043605
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Estrada-Gutierrez G
中科院分区：
文献类型：
--
作者：
Castro-Leyva V;Espejel-Nuñez A;Barroso G;Zaga-Clavellina V;Flores-Pliego A;Morales-Mendez I;Giono-Cerezo S;Walsh SW;Estrada-Gutierrez G
比较从有和没有亚临床宫内感染的早产妇女中分离的蜕膜细胞体外保存的炎症反应。35例自发性早产，孕37周前分娩，无宫内感染临床证据的妇女，剖宫产后取胎膜。对蜕膜进行微生物检测和培养。采用Bio-ELISA法检测细胞培养上清中抗炎细胞因子（IL-2、IL-4、IL-10）、促炎细胞因子（IL-6、IL-8、IL-1β、TNF-α）和基质金属蛋白酶（MMP-1、MMP-2、MMP-3、MMP-7、MMP-8、MMP-9）的浓度，采用酶免疫法检测前列腺素E2（PGE 2）的浓度。10名妇女（28.5%）证实存在亚临床感染。分离出的微生物为解脲脲原体（4）、B组链球菌（3）、迷走加德纳菌（1）和大肠埃希菌（2）。我们发现一个显着增加促炎细胞因子和抗炎细胞因子的显着减少，从蜕膜细胞的上清液中获得的妇女早产和亚临床宫内感染相比，妇女没有感染。感染组MMP-1、MMP-8、MMP-9和PGE 2的分泌量明显高于对照组。分泌IL-8的蜕膜细胞从感染的妇女坚持反复在体外培养传代。近30%的特发性早产病例与亚临床宫内感染有关，从这些病例中分离的蜕膜细胞在体内细菌暴露后保留了离体炎症状态。
To compare the inflammatory response preserved ex vivo by decidual cells isolated from women who experienced preterm labor with and without subclinical intrauterine infection. Fetal membranes were obtained after cesarean section from 35 women who delivered before 37 weeks of gestation following spontaneous preterm labor, with no clinical evidence of intrauterine infection. Decidua was microbiologically tested and cultured. Concentrations of anti-inflammatory cytokines (IL-2, IL-4, IL-10), pro-inflammatory cytokines (IL-6, IL-8, IL-1β and TNF-α), and matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9) were measured in the supernatants using Bio-Plex, and prostaglandin E2 (PGE2) was measured by enzyme immunoassay. Subclinical infection was confirmed in 10 women (28.5%). Microorganisms isolated were Ureaplasma urealyticum (4), group B streptococci (3), Gardnerella vaginalis (1), and Escherichia coli (2). We found a significant increase of pro-inflammatory cytokines and a significant decrease of anti-inflammatory cytokines in supernatants from decidual cells obtained from women with preterm labor and subclinical intrauterine infection compared to women without infection. Secretion of MMP-1, MMP-8, MMP-9 and PGE2 was significantly higher in infected women. Secretion of IL-8 by decidual cells from infected women persisted upon repeated in vitro culture passages. Almost 30% of idiopathic preterm labor cases were associated with subclinical intrauterine infection, and decidual cells isolated from these cases preserved an ex vivo inflammatory status after in vivo bacterial exposure.
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影响因子：
3.6
作者：
Arechavaleta-Velasco, F;Ogando, D;Vadillo-Ortega, F
通讯作者：
Vadillo-Ortega, F
DOI：
10.1902/annals.2001.6.1.153
发表时间：
2001-12-01
期刊：
Annals of periodontology
影响因子：
--
作者：
Gibbs, R S
通讯作者：
Gibbs, R S
影响因子：
4
作者：
Garcia-Lopez, Guadalupe;Vadillo-Ortega, Felipe;Zaga-Clavellina, Verónica
通讯作者：
Zaga-Clavellina, Verónica
影响因子：
3.8
作者：
MITCHELL, MD;EDWIN, SS;TRAUTMAN, MS
通讯作者：
TRAUTMAN, MS
影响因子：
3.8
作者：
Keelan, JA;Blumenstein, M;Mitchell, MD
通讯作者：
Mitchell, MD