Ion effects on the aggregation and DNA-binding reactions of Escherichia coli RNA polymerase.
Ion effects on the aggregation and DNA-binding reactions of Escherichia coli RNA polymerase.
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离子对大肠杆菌 RNA 聚合酶聚集和 DNA 结合反应的影响。
DOI：
10.1101/sqb.1983.047.01.055
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
RecordJr,MT
中科院分区：
文献类型：
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作者：
Shaner,SL;Melançon,P;Lee,KS;Burgess,RR;RecordJr,MT
材料和方法SRNA聚合酶。根据Burgess和Jendrisak(1975)的方法制备了柯尔特大肠杆菌K-12株的RNA聚合酶全酶，该方法由Lowe等人(1979)修改。根据Chamberlin等人(1979)的测定，该酶(87%o-饱和)的活性为66.10%。在最近的差异沉降速度和肝素竞争过滤结合实验中使用的全酶添加了纯化的o(Lowe等人。1979)，以产生Ao含量
MATERIALS AND METHODSRNA polymerase. RNA polymerase holoenzyme from the K-12 strain of E. colt was prepared according to the method of Burgess and Jendrisak (1975) as modified by Lowe et al.(1979). The enzyme (87% o-saturated) was found to be 66• 10% active, using the assay of Chamberlin et al.(1979). Holoenzyme used in the recent difference sedimentation velocity and heparin competition filter-binding experiments was supplemented with purified o (Lowe et al. 1979) to yield ao content of