Time-Restricted Feeding and Caloric Restriction: Two Feeding Regimens at the Crossroad of Metabolic and Circadian Regulation.
Time-Restricted Feeding and Caloric Restriction: Two Feeding Regimens at the Crossroad of Metabolic and Circadian Regulation.
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DOI：
10.1007/978-1-0716-2249-0_22
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发表时间：
2022
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除了饮食的质量和数量，食物摄入的“时间”最近成为营养和代谢健康的第三个关键参数。营养时间和代谢稳态之间的联系部分是由于昼夜节律钟对每日摄食的调节：禁食周期和代谢途径。啮齿动物的临床前喂养方案研究对于进一步确定这种关系的模式并更好地理解其机制基础是非常宝贵的。限时喂养（TRF）和热量限制（CR）是处于代谢和昼夜节律调节十字路口的喂养方案的例子。在这里，我们提出了实现TRF和CR的方法，突出了与昼夜节律和代谢健康研究相关的参数。我们还提供了方法来确定它们对生物钟输出的影响，通过使用24小时时间序列收集分析昼夜表达谱，以及使用葡萄糖耐量试验（GTT）来确定它们对代谢稳态的影响。
In addition to diet quality and quantity, the “timing” of food intake recently emerged as a third key parameter in nutritional and metabolic health. The link between nutrition timing and metabolic homeostasis is in part due to the regulation of daily feeding:fasting cycles and metabolic pathways by the circadian clock. Preclinical feeding regimen studies in rodents are invaluable to further define the modalities of this relationship and get a better understanding of its mechanistic underpinnings. Time-restricted feeding (TRF) and caloric restriction (CR) are examples of feeding regimen at the crossroads of metabolic and circadian regulation. Here we propose methods to implement TRF and CR highlighting the parameters that are relevant to the study of circadian and metabolic health. We also provide methods to determine their impact on the output of the circadian clock by analyzing diurnal expression profiles using 24 h time-series collection as well as their impact on metabolic homeostasis using a glucose tolerance test (GTT).