The role of adenosine for IgE receptor-dependent degranulation of human peripheral basophils and skin mast cells
The role of adenosine for IgE receptor-dependent degranulation of human peripheral basophils and skin mast cells
复制标题
腺苷对人外周嗜碱性粒细胞和皮肤肥大细胞 IgE 受体依赖性脱颗粒的作用
DOI：
10.1016/j.alit.2018.03.007
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发表时间：
2018
影响因子：
6.8
通讯作者：
Hide M
中科院分区：
文献类型：
--
作者：
Matsuo Y, Yanase Y;Irifuku R;Ishii K;Kawaguchi T;Takahagi S;Hide I;Hide M
嗜碱性粒细胞和肥大细胞(MC)在IgE抗体相关的变态反应性疾病中起着重要作用，如荨麻疹、花粉过敏和食物过敏。嗜碱性粒细胞和巨噬细胞表面表达高亲和力的ε受体(Fc、IgERI)。1，2抗原(变应原)使细胞表面的IgE发生交联反应，导致分泌颗粒释放预先形成的组胺等介质，继而产生新合成的介质，包括花生四烯酸代谢产物和细胞因子。虽然嗜碱性粒细胞和MC激活的机制已经被很好地研究，但这些细胞激活的衰减途径仍然不清楚。腺苷被认为是平衡炎症反应的内源性危难信号。3、4它是由三磷酸腺苷(ATP)经酶促磷酸水解制得，可激活所有四种G蛋白偶联的腺苷受体(A1、A2a、A2b和A3)。戈麦斯等人。报道称，高浓度(10±4e 10±3M)腺苷可抑制FcεRI诱导的人肺和皮肤肥大细胞脱颗粒。另一方面，其他研究表明，腺苷增强了人类肥大细胞系LAD2和小鼠骨髓来源的肥大细胞(BMMCs)对IgE依赖的脱颗粒。7.
Basophils and mast cells (MCs) play important roles in IgE antibody-associated allergic disorders, such as urticaria, pollen allergy and food allergy. Basophils and MCs express high-affinity IgE receptors (FcεRI) on their surface. 1, 2 Crosslinkage of IgE on the cell surface by antigen (allergen) results in the release of preformed mediators, such as histamine, from secretory granules, followed by generation of newly synthesized mediators including the products of arachidonic acid metabolism and cytokines. Although the mechanisms of basophil and MC activation have been well investigated, the attenuation pathway of the activation of these cells has remained unclear.Adenosine has been implicated as an endogenous distress signal to balance inflammatory responses. 3, 4 It is rapidly produced from adenosine triphosphate (ATP) by enzymatic phosphohydrolysis, and can activate all four G protein-coupled adenosine receptors (A1, A2a, A2b and A3). Gomez et al. reported that treatment with high concentrations (10 À4 e 10À3 M) of adenosine suppressed FcεRI-induced degranulation of human lung and skin mast cells. 5 On the other hand, other studies have shown that adenosine potentiates IgE-dependent degranulation of LAD2, a human mast cell line, 6 and mouse bone marrow derived mast cells (BMMCs). 7