PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats.
PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats.
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DOI：
10.1186/1475-9292-1-2
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发表时间：
2002-05-29
期刊：
影响因子：
--
通讯作者：
Cuny, Gerard
中科院分区：
文献类型：
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作者：
Desquesnes, Marc;Ravel, Sophie;Cuny, Gerard
锥虫（Herpetosoma）lewisi是锥虫属Herpetosoma亚属（Stercoraria部分）的一种锥虫，通过跳蚤传播大鼠（Rattus rattus和Rattus norvegicus）的寄生虫。T. Lewisi具有严格的物种特异性，并且不能在其它啮齿动物如小鼠中生长。大鼠主要通过经口途径感染，通过跳蚤粪便污染或摄入跳蚤。鼠群中的路易氏锥虫感染会干扰研究方案，而野鼠的跳蚤通常是此类感染的来源。目前，T.通过显微镜观察染色的血涂片来进行大鼠中的Lewisi。在CIRDES的一个研究项目中，一个T。在鼠群中检测到Lewisi感染。在这项研究中，我们评估了PCR引物集的能力，诊断多种锥虫与一个单一的扩增。我们发现，使用核糖体DNA的ITS1序列提供了一个有效和敏感的检测和鉴定T。lewisi感染的大鼠，并建议使用该测定监测T。大鼠群体中的路易氏感染。
Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas. T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Rats are infected principally by oral route, through contamination by flea faeces or ingestion of fleas. Trypanosoma lewisi infections in rat colonies can interfere with research protocols and fleas of wild rats are often the source of such infections. Currently, diagnosis of T. lewisi in rats is performed by microscopic observation of stained blood smears. In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony. In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification. We show that the use of ITS1 sequence of ribosomal DNA provides an efficient and sensitive assay for detection and identification of T. lewisi infection in rats and recommend the use of this assay for monitoring of T. lewisi infections in rat colonies.