Determination of Cry9C protein in processed foods made with StarLink corn.
Determination of Cry9C protein in processed foods made with StarLink corn.
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DOI：
10.1093/jaoac/85.5.1070
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发表时间：
2002-09
影响因子：
1.6
通讯作者：
C. Díaz;C. Fernández;R. McDonald;J. Yeung
中科院分区：
文献类型：
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作者：
C. Díaz;C. Fernández;R. McDonald;J. Yeung
StarLink（Aventis CropScience US）杂交玉米经过基因改造，含有一种杀虫蛋白Cry 9 C，这使得它比传统品种更能抵抗某些类型的玉米害虫。与其他转基因玉米品种不同，美国环境保护署授权将StarLink玉米仅用于动物饲料和工业用途，而不是人类消费。然而，一些含有Cry 9 C的玉米被错误地或无意中与用于人类食品的黄色玉米混合。由于含有Cry 9 C结构的玉米未被批准用于人类使用，美国食品和药物管理局认为含有它的食品是掺假的。因此，这一违规行为导致数百起含有cry 9 C DNA的玉米产品召回，如玉米卷壳。检测StarLink玉米中的新型蛋白质是一个新出现的问题;因此，没有标准化或建立的分析方法来检测加工食品中的Cry 9 C蛋白质。我们开发了一种用于定量加工食品基质中Cry 9 C蛋白的方法，并提供了验证数据，定量限为1.7 ng/g（ppb），使用基于多克隆抗体的商用Cry 9 C试剂盒，该试剂盒预期用于玉米谷物样品。批内和批间变异系数分别为2.8%和11.8%。在2 ng/g和5 ng/g Cry 9 C强化水平下，19种对照非StarLink玉米基质的平均回收率分别为73%和85%。我们的数据表明，只有0-0.5%的Cry 9 C蛋白在由100% StarLink玉米制成的玉米饼片和软玉米饼的加工中存活，导致水平从低于检测限到45 ppb。
StarLink (Aventis CropScience US) hybrid corn has been genetically modified to contain a pesticidal protein, Cry9C, which makes it more resistant than traditional varieties to certain types of corn insect pests. Unlike other varieties of genetically engineered corn, the U.S. Environmental Protection Agency authorized the use of StarLink corn for animal feed and industrial use only, not for human consumption. However, some Cry9C-containing corn was mistakenly or inadvertently comingled with yellow corn intended for human food use. Because corn containing the Cry9C construct was not approved for human use, the U.S. Food and Drug Administration considers food containing it to be adulterated. Consequently, this regulatory violation resulted in hundreds of recalls of corn-based products, such as taco shells, containing cry9C DNA. Detecting the novel protein in StarLink corn is an emerging issue; therefore, there is no standardized or established analytical method for detecting Cry9C protein in processed foods. We developed a procedure for quantitation of Cry9C protein, with validation data, in processed food matrixes with a limit of quantitation at 1.7 ng/g (ppb), using a commercial polyclonal antibody-based Cry9C kit that was intended for corn grain samples. Intra- and interassay coefficients of variation were 2.8 and 11.8%, respectively. Mean recoveries were 73 and 85% at 2 and 5 ng/g Cry9C fortifications, respectively, for 19 control non-StarLink corn-based matrixes. Our data demonstrate only 0-0.5% of Cry9C protein survived the processing of tortilla chips and soft tortillas made from 100% StarLink corn, resulting in levels from below the detection limit to 45 ppb.