Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan.
Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan.
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新认识的人类致病原生动物的培养和系统发育特征。
DOI：
10.1093/infdis/169.5.1050
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Persing,DH
中科院分区：
文献类型：
--
作者：
Thomford,JW;Conrad,PA;Telford3rd,SR;Mathiesen,D;Bowman,BH;Spielman,A;Eberhard,ML;Herwaldt,BL;Quick,RE;Persing,DH
最近从华盛顿州的一名病人身上分离到一种红细胞内原生动物（WA1），它在形态上与小鳍Babalus一致，但在生物学和遗传学上却截然不同。在静止红细胞培养物中建立了WA 1的连续生长。将发光巴贝虫特异性DNA探针与限制酶消化的基因组DNA的Southern印迹杂交显示，WA1可以与抗原性交叉反应的其他巴贝虫物种（吉氏巴贝虫和来自沙漠大角羊的巴贝虫寄生虫，Ovis canadensis nelsoni）或已知感染人类的巴贝虫物种（B. microti、分歧巴氏杆菌和马巴氏杆菌）或两者。对WA1的核小亚基rRNA基因的1436 bp片段进行了测序分析。遗传距离分析表明，WA1与犬病原菌B的亲缘关系最近。gibsoni和泰勒虫属（Theileriaspecies）和B.所描述的方法将有助于改进人类原生动物病原体的诊断和鉴定。
An intraerythrocytic protozoan (WA1) recently isolated from a patient in Washington State was shown to be morphologically identical toBabesia microtibut biologically and genetically distinct. Continuous growth of WA1 was established in stationary erythrocyte cultures. Hybridization of a chemiluminescentBabesia-specific DNA probe to Southern blots of restriction enzyme-digested genomic DNA showed that WA1 could be distinguished from other Babesia species that were antigenically cross-reactive (Babesia gibsoniand babesial parasites from desert bighorn sheep,Ovis canadensis nelsoni) or known to infect humans (B. microti,Babesia divergens, andBabesia equi), or both. A 1436-bp portion of the nuclear small subunit rRNA gene of WA1 was sequenced and analyzed. Genetic distance analysis showed that WA1 is most closely related to the canine pathogenB. gibsoniand lies within a phylogenetic cluster withTheileriaspecies andB. equi.The methodology described will be useful for improved diagnosis and identification of human protozoal pathogens.