Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles.
Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles.
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DOI：
10.1371/journal.pone.0130965
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wilson CC
中科院分区：
文献类型：
--
作者：
Davy CM;Kidd AG;Wilson CC
环境DNA（eDNA）是一种潜在的强有力的工具，用于检测和监测稀有物种，包括受威胁的本地物种和最近到达的入侵物种。在这里，我们开发了一套9个同域淡水龟的DNA引物，并使用它来测试是否可以成功地检测到海龟eDNA从水族馆和室外池塘的样品。我们还使用目标区域两个地点的实地调查数据，对eDNA检测和通过传统调查方法进行检测的成本进行了比较。我们发现，海龟的eDNA可以使用传统的聚合酶链反应（PCR）和定量PCR（qPCR）进行检测，并且通过传统的调查方法进行检测的成本比我们研究范围内的物种的eDNA检测高2- 10倍。我们总结了未来必要的步骤，应用eDNA调查海龟监测和保护，并提出了具体的情况下，eDNA的应用可以进一步保护受威胁的海龟物种。
Environmental DNA (eDNA) is a potentially powerful tool for detection and monitoring of rare species, including threatened native species and recently arrived invasive species. Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can be successfully detected in samples from aquaria and an outdoor pond. We also conduct a cost comparison between eDNA detection and detection through traditional survey methods, using data from field surveys at two sites in our target area. We find that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2–10X higher than eDNA detection for the species in our study range. We summarize necessary future steps for application of eDNA surveys to turtle monitoring and conservation and propose specific cases in which the application of eDNA could further the conservation of threatened turtle species.