PROLIFERATIVE PATTERN OF UTERINE CELLS FROM BIRTH TO ADULTHOOD IN INTACT, NEONATALLY CASTRATED, AND OR ADRENALECTOMIZED MICE, ASSAYED BY INCORPORATION OF [IODODEOXYURIDINE-I-125
PROLIFERATIVE PATTERN OF UTERINE CELLS FROM BIRTH TO ADULTHOOD IN INTACT, NEONATALLY CASTRATED, AND OR ADRENALECTOMIZED MICE, ASSAYED BY INCORPORATION OF [IODODEOXYURIDINE-I-125
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DOI：
10.1210/endo-113-2-582
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发表时间：
1983-01-01
期刊：
影响因子：
4.8
通讯作者：
MATSUMOTO, K
中科院分区：
文献类型：
--
作者：
OGASAWARA, Y;OKAMOTO, S;MATSUMOTO, K
在小鼠身上研究了子宫细胞从出生到成年的增殖模式。整个子宫对5-[125I]碘-2''-脱氧尿苷([125I]IdUrd)的摄取被用作细胞增殖的指标。在完整小鼠和新生阉割小鼠中，出生后 0-25 天的子宫生长发生了几乎平行的变化。两组小鼠的子宫重量均增加（0.7-6 mg），并且在第 0-15 天发现[125I]IdUrd 摄取值较高，而体重几乎保持不变，并且在接下来的 10 天发现摄取值非常低。肾上腺切除加卵巢切除对新生儿子宫生长没有显着影响。第25天后，完整小鼠的子宫重量（6-50毫克）和[125I]IdUrd摄取再次增加，但新生去势小鼠的子宫重量仍然较低。然后检查子宫细胞对外源17β-雌二醇的增殖反应。在第0天(20μg/小鼠)和第10天(5μg/小鼠)注射17.β-雌二醇诱导第二天[125I]IdUrd摄取显着增加。新生儿去势对成年小鼠子宫对雌激素诱导的生长的反应没有显着影响。新生和青春期前小鼠的卵巢和肾上腺分泌的性类固醇显然对子宫细胞的增殖没有显着作用，并且细胞增殖的静止期出现在出生后第20天左右，介于小鼠子宫细胞的自主增殖（第0-15天）和卵巢依赖性增殖（第25天之后）之间。
The proliferative pattern of uterine cells from birth to adulthood was investigated in mice. The uptake of 5-[125I]iodo-2''-deoxyuridine ([125I]IdUrd) by the whole uterus was used as an index of cell proliferation. Nearly parallel changes in uterine growth were found from days 0-25 after birth in both intact and neonatally castrated mice. In both groups of mice, the weight of the uterus increased (0.7-6 mg) and high [125I]IdUrd uptake values were found from days 0-15, while the weight remained nearly constant, and very low uptake values were found in the next 10 days. Adrenalectomy plus ovariectomy caused no significant effect on neonatal growth of the uterus. After day 25, the weight of uterus (6-50 mg) and [125I]IdUrd uptake increased again in the intact mice, but remained low in the neonatally castrated mice. The proliferative response of the uterine cells to exogenous 17.beta.-estradiol was then examined. The injection of 17.beta.-estradiol on days 0 (20 .mu.g/mouse) and 10 (5 .mu.g/mouse) induced significant increases in [125I]IdUrd uptake the next day. Neonatal castration had no significant effect on the responsiveness of the uterus to estrogen-induced growth in adult mice. Sex steroids secreted from both the ovaries and adrenals of neonatal and prepubertal mice apparently play no significant role in the proliferation of uterine cells, and a quiescent interval of cell proliferation occurs around day 20 after birth between the autonomous (days 0-15) and the ovary-dependent (after day 25) proliferation of mouse uterine cells.