DNA damage induced by ethoxyquin in human peripheral lymphocytes
DNA damage induced by ethoxyquin in human peripheral lymphocytes
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DOI：
10.1016/j.toxlet.2005.09.040
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发表时间：
2006-05-05
影响因子：
3.5
通讯作者：
Blaszczyk, A
中科院分区：
文献类型：
--
作者：
Blaszczyk, A
乙氧基喹啉（1，2-二氢-6-乙氧基-2，2，4-三甲基喹啉，EQ）因其强大的抗氧化活性而广泛用于各种食品和动物饲料中。最近发现，这种化合物不仅对喂食含有它的饲料的动物造成许多不利的副作用，而且对在工作中接触它的人也产生不利影响。在本研究中，EQ诱导的人淋巴细胞的DNA损伤进行了评估。采用碱性单细胞凝胶电泳法（彗星试验）检测DNA损伤。在不存在或存在外源性代谢活化系统（S9混合物）的情况下，用1至250 μ M的EQ剂量处理细胞1小时。所得结果表明，EQ以剂量依赖性方式诱导人淋巴细胞的DNA损伤;与相同剂量的EQ单独处理的细胞相比，在存在代谢活化系统的情况下观察到的EQ诱导的DNA片段化始终显著较低。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Ethoxyquin (1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline, EQ) is widely used in various food products and in animal feeds because of its powerful antioxidant activity. This compound was recently found to cause not only many unfavourable side-effects in animals fed with feeds containing it, but also adverse effects in people exposed to it at work. In the present study, DNA damage induced by EQ in human lymphocytes has been assessed. The alkaline single cell gel electrophoresis assay (comet assay) was used to measure DNA damage. The cells were treated for I h with EQ doses ranging from I to 250 mu M in the absence or in the presence of an exogenous metabolic activation system (S9mix). The obtained results showed that EQ-induced DNA damage in human lymphocytes in a dose-dependent manner; the observed DNA fragmentation induced by EQ in the presence of metabolic activation system was always significantly lower, as compared to cells treated with the same doses of EQ alone. (c) 2005 Elsevier Ireland Ltd. All rights reserved.