Transduction of a cellular oncogene: the genesis of Rous sarcoma virus.
Transduction of a cellular oncogene: the genesis of Rous sarcoma virus.
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细胞癌基因的转导：劳斯肉瘤病毒的起源。
DOI：
10.1073/pnas.80.9.2519
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发表时间：
1983
影响因子：
11.1
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Swanstrom,R;Parker,RC;Varmus,HE;Bishop,JM
劳斯肉瘤病毒的癌基因（v-src）是由细胞基因（c-src）转导产生的。为了探索转导机制，我们已经确定了v-src mRNA发生中使用的剪接受体位点，表明c-src mRNA剪接中使用了等效位点，并从v-src和c-src之间的同源性边界确定了核苷酸序列。我们的数据表明：（i）只有一部分c-src在v-src中出现，（ii）转导病毒基因组和c-src之间的重组发生在细胞基因的内含子中，（iii）v-src部分是c-src相应部分的剪接版本，（iv）在转导病毒基因组中出现一次的核苷酸序列变成复制到v-src的侧翼。这些发现表明转导的第一步可能是转导病毒基因组的DNA形式与c-src之间的重组，并且另外支持逆转录病毒转导的流行模型。由v-src和c-src编码的蛋白质的羧基末端明显不同。127或128个核苷酸的未鉴定结构域位于两种RSV株基因组中的不同位置，并提供了通过不依赖于src转导的遗传转座进入病毒基因组的外源因子的证据。
The oncogene of Rous sarcoma virus (v-src) arose by transduction of a cellular gene (c-src). In an effort to explore the mechanism of transduction, we have identified the splice acceptor site used in the genesis of mRNA for v-src, shown that an equivalent site is used in the splicing of mRNA for c-src, and determined the nucleotide sequence from the boundaries of homology between v-src and c-src. Our data indicate that (i) only a portion of c-src is represented within v-src, (ii) the leftward recombination between the genome of the transducing virus and c-src occurred in an intron of the cellular gene, (iii) v-src is in part a spliced version of the corresponding portion of c-src, and (iv) nucleotide sequences represented once in the genome of the transducing virus become duplicated to flank v-src. These findings indicate that the first step in transduction is probably recombination between DNA forms of the transducing viral genome and c-src and otherwise support the prevailing model for transduction by retroviruses. The carboxyl termini of the proteins encoded by v-src and c-src differ appreciably. An unidentified domain of 127 or 128 nucleotides is located at different positions in the genomes of two strains of RSV and gives evidence of being a foreign element that entered the viral genomes by genetic transposition independent of the transduction of src.