Efficient Retention and Complexation of Exogenous Ferulic Acid in Starch: Could Controllable Bioextrusion Be the Answer?
Efficient Retention and Complexation of Exogenous Ferulic Acid in Starch: Could Controllable Bioextrusion Be the Answer?
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DOI：
10.1021/acs.jafc.2c04261
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发表时间：
2022-11
影响因子：
6.1
通讯作者：
Shuohan Ma;Qingqing Zhu;Siyu Yao;Ruihao Niu;Yu Liu;Yu Qin;Yuxue Zheng;Jinhu Tian;Dandan Li
中科院分区：
文献类型：
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作者：
Shuohan Ma;Qingqing Zhu;Siyu Yao;Ruihao Niu;Yu Liu;Yu Qin;Yuxue Zheng;Jinhu Tian;Dandan Li
淀粉-酚类复合体作为功能食品被广泛应用，但传统的液体反应和洗涤体系限制了淀粉-酚类化合物的保留率。在本研究中，阿魏酸(FA，5%)以晶型外源使用，并在高固相挤压环境中与淀粉反应，同时受热稳定的α-淀粉酶(0-252U/g)控制。适度酶解(21U/g或63U/g)降低了淀粉的双螺旋程度，显著提高了脂肪酸的保留率(>；80%)，且具有良好的熔融和分布。虽然没有明显的强化学键(只有0.17-2.39%的FA与淀粉水解物结合)，但通过1H核磁共振和分子动力学模拟分析，确定了非共价相互作用，主要是氢键、范德华力和静电相互作用。在体外消化条件下，促进了生物挤出物中总FA(胃中50%，肠中∼100%)的阶段性释放，为处理大负荷的FA和其他以淀粉为载体的酚类物质提供了前景。
The starch-phenolics complexes are widely fabricated as functional foods but with low phenolics retention limited by traditional liquid reaction and washing systems. In this study, ferulic acid (FA, 5%) was exogenously used in the crystalline form, and it reacted with starch in a high-solid extrusion environment, which was simultaneously controlled by thermostable α-amylase (0-252 U/g). Moderate enzymolysis (21 or 63 U/g) decreased the degree of the starch double helix and significantly increased the FA retention rate (>80%) with good melting and distribution. Although there were no significantly strong chemical bonds (with only 0.17-2.39% FA bound to starch hydrolysate), the noncovalent interactions, mainly hydrogen bonds, van der Waals forces, and electrostatic interactions, were determined by 1H NMR and molecular dynamics simulation analyses. The phased release of total FA (>50% in the stomach and ∼100% in the intestines) from bioextrudate under in vitro digestion conditions was promoted, which gives a perspective for handing large loads of FA and other phenolics based on starch carrier.