Protein phosphatase 1, but not protein phosphatase 2A, dephosphorylates DNA-damaging stress-induced phospho-serine 15 of p53
Protein phosphatase 1, but not protein phosphatase 2A, dephosphorylates DNA-damaging stress-induced phospho-serine 15 of p53
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DOI：
10.1016/j.febslet.2004.04.066
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发表时间：
2004-06-04
期刊：
影响因子：
3.5
通讯作者：
Isobe, K
中科院分区：
文献类型：
--
作者：
Haneda, M;Kojima, E;Isobe, K
冈田酸（OA）是一种蛋白磷酸酶（PP）抑制剂，可诱导p53的过度磷酸化。我们研究了OA对PP1的抑制是否促进了p53丝氨酸15的磷酸化。体外去磷酸化实验表明，PP1可使紫外线C （UVC）诱导的p53磷酸化-ser15去磷酸化，OA对其有抑制作用。其中一种PP1调控因子生长阻滞和DNA损伤34 （growth arrest and DNA damage 34, GADD34）干扰PP1与p53的结合，干扰p53的去磷酸化，并在uvc处理后增加磷酸化p53的数量。该报告提供了PP1而不是PP2A使p53的磷酸丝氨酸15去磷酸化的第一个证据。(C) 2004年欧洲生化学会联合会。Elsevier B.V.版权所有。
Okadaic acid (OA) is a protein phosphatase (PP) inhibitor and induces hyperphosphorylation of p53. We investigated whether the inhibition of PP1 by OA promotes the phosphorylation of the serine 15 of p53. In vitro dephosphorylation assay showed that PP1 dephosphorylated ultraviolet C (UVC)-induced phospho-ser15 of p53, and that OA treatment inhibited it. One of the PP1 regulators, growth arrest and DNA damage 34 (GADD34), disturbed PP1 binding with p53, interfered with the dephosphorylation of p53 and increased the amount of phospho-p53 after UVC-treatment. This report provides the first evidence that PP1, but not PP2A, dephosphorylates phospho-serine 15 of p53. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.