Synergistic inhibition of anaerobic ammonium oxidation (anammox) activity by phenol and thiocyanate.
Synergistic inhibition of anaerobic ammonium oxidation (anammox) activity by phenol and thiocyanate.
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DOI：
10.1016/j.chemosphere.2018.09.055
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发表时间：
2018-09
期刊：
影响因子：
8.8
通讯作者：
M. Oshiki;Y. Masuda;Takashi Yamaguchi;N. Araki
中科院分区：
文献类型：
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作者：
M. Oshiki;Y. Masuda;Takashi Yamaguchi;N. Araki
钢铁生产过程中排放的焦化废水是一种富含酚和硫氰酸盐的废水，对废水生物处理过程中的微生物活性具有抑制作用。在本研究中，协同抑制厌氧氨氧化（anammox）活性的苯酚和SCN的厌氧氨氧化反应器的间歇培养和连续运行进行了研究。在分批培养中测定的厌氧氨氧化活性的比较表明，苯酚和SCN的协同抑制作用大于苯酚或SCN单独的抑制作用，其中厌氧氨氧化生物质与10-250 mg L-1的i）苯酚、ii）SCN或iii）苯酚和SCN一起缺氧培养。通过在上流式厌氧氨氧化反应器中连续运行262 d，进一步考察了苯酚和SCN的协同抑制作用。当进水中苯酚和SCN的浓度分别增加到16和32 mg L−1时，NH 4+和NO2−的去除效率下降，并通过15 NO2 −示踪实验进一步研究了厌氧氨氧化酶活性的抑制。苯酚和SCN的加入导致厌氧氨氧化菌的种群发生了变化，优势种由“土瓜鱼”变为“Ca.锦芋固氮菌和硫杆菌16 S rRNA基因片段的相对丰度在运行过程中有所增加，表明它们是苯酚和SCN厌氧降解的主要原因。本研究是第一个文件的协同抑制anammox活性苯酚和SCN和微生物财团参与脱氮以及苯酚和SCN降解。
Coke-oven wastewater discharged from the steel-manufacturing process is phenol and thiocyanate (SCN)-rich wastewater, which inhibits microbial activities in biological wastewater treatment processes. In the present study, synergistic inhibition of anaerobic ammonium oxidation (anammox) activity by phenol and SCN was examined by batch incubation and continuous operation of an anammox reactor. The comparison of anammox activities determined in the batch incubation, in which the anammox biomass was anoxically incubated with 10–250 mg L−1of i) phenol, ii) SCN, or iii) both phenol and SCN, showed that synergistic inhibition by phenol and SCN was greater than the inhibitions by phenol or SCN alone. The synergistic inhibition by phenol and SCN was further investigated by operating an up-flow column anammox reactor for 262 d. The removal efficiencies of NH4+and NO2−deteriorated when phenol and SCN concentrations in the influent increased to 16 and 32 mg L−1, respectively, and the inhibition of anammox activity was further investigated by a15NO2−tracer experiment. Addition of phenol and SCN resulted in a population shift of anammox bacteria, and the dominant species changed from “CandidatusKuenenia stuttgartiensis” to “Ca. Brocadia sinica”. The relative abundance ofAzoarcusandThiobacillus16S rRNA gene reads increased during the operation, suggesting that they were responsible for the anaerobic phenol and SCN degradation. The present study is the first to document the synergistic inhibition of anammox activity by phenol and SCN and the microbial consortia involved in the nitrogen removal as well as the phenol and SCN degradations.