Overexpression of TaSRK2C1, a Wheat SNF1-Related Protein Kinase 2 Gene, Increases Tolerance to Dehydration, Salt, and Low Temperature in Transgenic Tobacco
Overexpression of TaSRK2C1, a Wheat SNF1-Related Protein Kinase 2 Gene, Increases Tolerance to Dehydration, Salt, and Low Temperature in Transgenic Tobacco
复制标题
TaSRK2C1（一种小麦 SNF1 相关蛋白激酶 2 基因）的过表达可提高转基因烟草对脱水、盐和低温的耐受性
DOI：
10.1007/s11105-012-0548-x
复制
发表时间：
2013-08-01
影响因子：
2.1
通讯作者：
Xiao, Kai
中科院分区：
文献类型：
--
作者：
Du, Xiaoming;Zhao, Xiaolei;Xiao, Kai
蛋白质磷酸化-去磷酸化是植物在渗透胁迫下发生的主要信号转导事件。在这项研究中，小麦SNF 1相关蛋白激酶2（SnRK 2）基因，TaSRK 2C 1，功能的特点。序列分析结果表明，TaSRK 2C 1含有SnRK 2蛋白激酶的典型保守结构域，包括ATP结合位点、N-豆蔻酰化位点、蛋白激酶激活信号和跨膜区。TaSRK 2C 1基因在根中的转录受干旱、高盐、低温和外源脱落酸的诱导，表明其在渗透胁迫信号转导中具有潜在的作用。TaSRK 2C 1在烟草中的异位表达显著上调了三个假定的中枢调节因子的表达水平，即RD 29 a，DREB 1A和DREB 2，它们参与了对渗透胁迫的响应。因此，在转基因植物中检测到更高水平的游离脯氨酸和可溶性碳水化合物，并赋予植物对高盐、脱水胁迫和低温的耐受性。研究结果表明，TaSRK 2C 1在植物对渗透胁迫的响应和适应中具有重要的功能，它通过介导不同非生物胁迫引起的信号转导而发挥作用。操纵TaSRK 2C 1以提高作物植物的抗逆性是可行的。
Protein phosphorylation-dephosphorylations are major signaling events induced by osmotic stress in plants. In this study, a wheat SNF1-related protein kinase 2 (SnRK2) gene, TaSRK2C1, was functionally characterized. The results from the sequence analysis showed that TaSRK2C1 contains conserved domains typified in SnRK2 protein kinases, including the ATP binding site, N-myristoylation site, protein kinase-activating signature, and transmembrane-spanning region. The transcripts of TaSRK2C1 in roots were induced by treatments of dehydration, high salinity, low temperature, and exogenous abscisic acid, which suggest its potential roles relative to osmotic stress signal transductions. The ectopic expression of TaSRK2C1 in tobacco significantly up-regulated the expression levels of three putative central regulators, namely, RD29a, DREB1A, and DREB2, which are involved in responding to osmotic stresses. Thus, higher levels of free proline and soluble carbohydrates in transgenic plants were detected, and conferred tolerance to high salinity, dehydration stress, and low temperature in plants. The overall results in this study indicate that TaSRK2C1 have important functions in plant response and adaptation to osmotic stresses via mediation of signal transductions initiated by distinct abiotic stresses. Manipulating TaSRK2C1 toward improving the osmotic-stress tolerance in crop plants is feasible.