USE OF PHOA GENE FUSIONS TO IDENTIFY A PILUS COLONIZATION FACTOR COORDINATELY REGULATED WITH CHOLERA-TOXIN
USE OF PHOA GENE FUSIONS TO IDENTIFY A PILUS COLONIZATION FACTOR COORDINATELY REGULATED WITH CHOLERA-TOXIN
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DOI：
10.1073/pnas.84.9.2833
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
MEKALANOS, JJ
中科院分区：
文献类型：
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作者：
TAYLOR, RK;MILLER, VL;MEKALANOS, JJ
转座子 TnphoA 用于在 phoA、碱性磷酸酶 (PhoA) 基因和编码霍乱弧菌分泌的蛋白质的基因之间产生融合。分离出的一种 PhoA+ 突变体在乳鼠肠道中的定殖能力显着降低。该突变体不再产生 20.5 kDa 蛋白 (TcpA)，我们证明该蛋白是霍乱弧菌菌毛的主要亚基。 TcpA 菌毛亚基的氨基末端序列分析表明，它与其他几种病原菌产生的菌毛蛋白具有氨基酸同源性。 TcpA菌毛在各种培养条件下与霍乱毒素协同表达，并且这种效应似乎依赖于toxR基因编码的转录激活因子。我们得出结论，toxR 基因在霍乱弧菌多个毒力基因的转录调控中发挥着核心作用。
The transposon TnphoA ws used to generate fusions between phoA, the gene for alkaline phosphatase (PhoA), and genes encoding proteins that are secreted by Vibrio cholerae. One of the PhoA+ mutants isolated showed a dramatic reduction in its ability to colonize the intestines of suckling mice. This mutant no longer produced a 20.5-kDa protein (TcpA) that we show is the major subunit of a V. cholerae pilus. Amino-terminal sequence analysis of the TcpA pilus subunit showed that it shares amino acid homology with the pilins produced by several other pathogenic bacteria. The TcpA pilus was coordinately expressed with cholera toxin under various culture conditions, and this effect appeared to be dependent on the transcriptional activator encoded by the toxR gene. We conclude that the toxR gene plays a central role in the transcriptional regulation of multiple virulence genes of V. cholerae.