Real-Time Monitoring of Peptide Cleavage Using a Nanopore Probe
Real-Time Monitoring of Peptide Cleavage Using a Nanopore Probe
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DOI：
10.1021/ja9004893
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发表时间：
2009-05-13
影响因子：
15
通讯作者：
Guan，Xiyun
中科院分区：
文献类型：
--
作者：
Zhao，Qitao;de Zoysa，Ranulu Samanthi S.;Guan，Xiyun
在这里，我们报告了一种快速、无标记的监测肽裂解的方法。在酶不存在和存在的情况下通过工程离子通道监测肽易位，可以获得有关酶促过程的定量化学动力学信息。除了在疾病诊断和药物发现方面的潜在应用之外，这种肽/蛋白质裂解方法还有望进一步发展为一种新型快速、无标记蛋白质测序技术。
Here we report a rapid, label-free method for monitoring peptide cleavage. Monitoring peptide translocation through an engineered ion channel in the absence and the presence of an enzyme allowed quantitative chemical kinetics information on enzymatic processes to be obtained. In addition to its potential application in disease diagnostics and drug discovery, this peptide/protein cleavage approach is envisioned for further development as a novel rapid, label-free protein sequencing technique.