Effect of curcumin on the adhesion of platelets to brain microvascular endothelial cells in vitro
Effect of curcumin on the adhesion of platelets to brain microvascular endothelial cells in vitro
复制标题
DOI：
10.1111/j.1745-7254.2008.00813.x
复制
发表时间：
2008-07
影响因子：
8.2
通讯作者：
Li Zhang;Z. Gu;Zheng-Hong Qin;Zhong-Qin Liang
中科院分区：
文献类型：
--
作者：
Li Zhang;Z. Gu;Zheng-Hong Qin;Zhong-Qin Liang
目的：探讨姜黄素对血小板与体外培养的脑微血管内皮细胞（BMEC）粘附的抑制作用。方法：将[3 H]腺嘌呤标记的血小板与BMEC共同孵育，观察姜黄素对血小板与BMEC粘附的影响。通过液体闪烁光谱法测定粘附于BMEC单层的血小板数量。凝血酶诱导的血小板P-选择素，糖蛋白IIb（GPIIb），和糖蛋白IIIa（GPIIIa）的细胞表面上的表达，通过流式细胞术测量。RT-PCR检测BMEC中P-选择素mRNA的表达。结果：凝血酶激活的血小板与正常BMEC之间、TNF-α激活的BMEC与正常血小板之间的粘附均显著增加，姜黄素（30-240 μmol/L）可抑制凝血酶激活的血小板与正常BMEC之间的粘附，并呈浓度依赖性。凝血酶激活的血小板和TNF-α刺激的BMEC表达P-选择素和E-选择素，姜黄素预处理30 min可抑制血小板和BMEC表达P-选择素和E-选择素，并呈剂量依赖性。结论：姜黄素可抑制血小板对BMEC的诱导作用，并呈浓度依赖性。这种作用可能与血小板和BMEC上P-选择素、E-选择素和GPIIb/GPIIIa表达减少有关。
AbstractAim:To determine whether curcumin prevents the adhesion of platelets to brain microvascular endothelial cells (BMECs) cultured in vitro.Methods:[3H]Adenine-labeled platelets were incubated with BMECs to investigate the role of curcumin in the adhesion of platelets to BMECs. The number of platelets adhering to the BMECs monolayer was determined by liquid scintillation spectroscopy. The thrombin-induced expression of platelets P-selectin, glycoprotein IIb (GPIIb), and glycoprotein IIIa (GPIIIa) on the cell surface, was measured by flow cytometry. P-selectin mRNA levels of BMECs were determined by RT-PCR. The TNF-α-induced expressions of P-selectin and E-selectin on the surface of BMECs were determined by Western blotting.Results:The adhesion between thrombin-activated platelets and normal BMECs, and that of TNF-α-activated BMECs and normal platelets were significantly increased, and this increase could be inhibited by curcumin (30–240 μmol/L) in a concentration-dependant manner. The platelets activated with thrombin and BMECs stimulated by TNF-α demonstrated an upregulated expressions of P-selectin and E-selectin, and this increase, when pretreated with curcumin for 30 min, could be restrained dose dependently. Curcumin also inhibited the increase of the GPIIb/GPIIIa expression of thrombin-activated platelets in a concentration-dependent manner.Conclusion:Curcumin can inhibit the platelets to BMECs. This effect may be related to the decreased expressions of P-selectin, E-selectin, and GPIIb/GPIIIa on platelets and BMECs.