Nanoplastics impact the zebrafish (Danio rerio) transcriptome: Associated developmental and neurobehavioral consequences.
Nanoplastics impact the zebrafish (Danio rerio) transcriptome: Associated developmental and neurobehavioral consequences.
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DOI：
10.1016/j.envpol.2020.115090
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发表时间：
2020-07
影响因子：
8.9
通讯作者：
A. F. Pedersen;Danielle N. Meyer;A. Petriv;Abraham L Soto;Jeremiah N. Shields;Camille Akemann;
中科院分区：
文献类型：
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作者：
A. F. Pedersen;Danielle N. Meyer;A. Petriv;Abraham L Soto;Jeremiah N. Shields;Camille Akemann;
微塑料（MP）是一种普遍存在的污染物，不仅在海洋和淡水水体中检测到，而且在世界各地的自来水和瓶装水中也检测到。虽然MP已被广泛研究，但其较小的对应物纳米塑料（NP）的毒性尚未得到很好的记录。尽管人类和动物可能大规模暴露于NP，但相关的健康风险仍不清楚，特别是在早期发育阶段。为了解决这个问题，我们研究了暴露于50和200纳米聚苯乙烯纳米颗粒对斑马鱼幼鱼健康的影响。从受精后6至120小时（hpf），发育中的斑马鱼暴露于一系列荧光NP（10- 10，000 ppm）。在暴露的幼鱼中发现累积量呈剂量依赖性增加，这可能与游泳过度活跃所证明的行为反应改变相一致。值得注意的是，暴露不会影响死亡率、孵化率或畸形;然而，转录组学分析表明，在高浓度和低浓度下都会出现神经变性和运动功能障碍。此外，这项研究的结果表明，NP可以在斑马鱼幼虫的组织中积累，改变它们的转录组，影响行为和生理，可能会降低受污染生态系统中的生物适应性。在关键的发展窗口期，这项研究的独特广泛规模提供了NP对人类和动物健康影响的关键多维表征。
Microplastics (MPs) are a ubiquitous pollutant detected not only in marine and freshwater bodies, but also in tap and bottled water worldwide. While MPs have been extensively studied, the toxicity of their smaller counterpart, nanoplastics (NPs), is not well documented. Despite likely large-scale human and animal exposure to NPs, the associated health risks remain unclear, especially during early developmental stages. To address this, we investigated the health impacts of exposures to both 50 and 200 nm polystyrene NPs in larval zebrafish. From 6 to 120 h post-fertilization (hpf), developing zebrafish were exposed to a range of fluorescent NPs (10-10,000 parts per billion). Dose-dependent increases in accumulation were identified in exposed larval fish, potentially coinciding with an altered behavioral response as evidenced through swimming hyperactivity. Notably, exposures did not impact mortality, hatching rate, or deformities; however, transcriptomic analysis suggests neurodegeneration and motor dysfunction at both high and low concentrations. Furthermore, results of this study suggest that NPs can accumulate in the tissues of larval zebrafish, alter their transcriptome, and affect behavior and physiology, potentially decreasing organismal fitness in contaminated ecosystems. The uniquely broad scale of this study during a critical window of development provides crucial multidimensional characterization of NP impacts on human and animal health.