A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l).
A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l).
复制标题
与 RNA 聚合酶 II 延伸因子同源的新型 cDNA 映射到人类染色体 5q31 (TCEB1L) 和小鼠染色体 11 (Tceb1l)。
作者：
J. Sowden;K. Morrison;J. Schofield;W. Putt;Y. Edwards
在mRNA链延长过程中，辅助RNA聚合酶II的辅助因子很少被鉴定。我们从小鼠和人类来源中分离出了一种新型cDNA Tceb 1 l，它编码一种163个氨基酸的蛋白质，并与最近发现的RNA聚合酶II转录延伸因子p15表现出显着的同一性。Tceb 1 l在整个脊椎动物中高度保守，并定位于小鼠11号染色体和人类染色体5 q31的同线区。Tceb 1 l在早期小鼠胚胎中显示出有限的表达模式，在那里它不存在于神经外胚层;后来Tceb 1 l在神经管的尾部区域表达，随后在许多组织中广泛表达，包括脑和脊髓。这些观察结果与Tceb 1 l是RNA聚合酶II延伸因子一致，并表明Tceb 1 l/p15样肽可能是影响RNA延伸的蛋白质的新家族。
Few of the auxiliary factors that assist RNA polymerase II in the process of mRNA chain elongation have been identified. We have isolated a novel cDNA, Tceb1l, from mouse and human sources that encodes a 163-amino-acid protein and shows a significant level of identity with a recently identified RNA polymerase II transcription elongation factor, p15. Tceb1l is highly conserved throughout vertebrates and maps to mouse chromosome 11 and to the syntenic region of human chromosome 5q31. Tceb1l shows a restricted pattern of expression in the early mouse embryo, where it is absent from the neurectoderm; later Tceb1l is expressed in the caudal region of the neural tube, followed by widespread expression in many tissues, including the brain and spinal cord. These observations are consistent with Tceb1l being an RNA polymerase II elongation factor and suggest that Tceb1l/p15-like peptides may be a new family of proteins that influence RNA elongation.