Supplementation of the thawing medium with reduced glutathione improves function of frozen-thawed goat spermatozoa
Supplementation of the thawing medium with reduced glutathione improves function of frozen-thawed goat spermatozoa
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DOI：
10.1016/j.repbio.2013.01.174
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发表时间：
2013-03-01
影响因子：
2.1
通讯作者：
Carlos Gardon, Juan
中科院分区：
文献类型：
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作者：
Gadea, Joaquin;Gumbao, David;Carlos Gardon, Juan
精子冷冻保存在山羊生产中是一种有效的生殖管理工具。然而，冷冻和解冻会对精子膜产生物理和化学压力，从而降低精子的活力和受精能力。本研究首先研究了在解冻延长剂中添加还原型谷胱甘肽(GSH，1 mM和5 mM)对山羊精子冷冻解冻后参数的影响。我们使用了一套功能性精子测试，包括计算机辅助精液分析(CASA)检测的精子活力、膜脂堆积障碍、自发性顶体反应、自由基产生(ROS产生)和精子染色质凝集。本研究的主要发现是：(1)在解冻液中添加GSH后，精子的活力和前进活力增加；(2)非获能精子数增加；(3)顶体完整的存活精子数增加；(4)ROS生成减少；(5)染色质凝集减少。在第二项研究中，评估了在解冻液中添加还原型谷胱甘肽(GSH，5 mM)或氧化型谷胱甘肽(GSSG，2.5 mM)的情况。证实了GSH对精子功能的保护作用。与GSH相比，在解冻液中添加GSSG对精子功能的保护作用较弱。在解冻稀释液中添加GSH可显著提高山羊冷冻精子的功能和受精能力。从这项研究中得到的信息表明，氧化应激是导致精子冷冻损伤的重要原因，并为探索抗氧化剂的实际应用以提高山羊解冻后精液的质量打开了新的窗口。(C)2013年生殖生物学学会&位于Olsztyn的波兰科学院动物生殖和食品研究所。出版：Elsevier Urban&Partner Sp.ZO.O。版权所有。
Sperm cryopreservation represents a useful tool in the management of reproduction in goat production. However, freezing and thawing produce physical and chemical stress on the sperm membrane that reduces their viability and fertilizing ability. In this study, firstly we evaluated the effects of reduced glutathione (GSH, 1 and 5 mM) supplementation of the thawing extender on parameters of frozen-thawed goat spermatozoa. We used a set of functional sperm tests that included sperm motility assayed by computer-assisted semen analysis (CASA), membrane lipid packing disorder, spontaneous acrosome reaction, free radical production (ROS generation) and sperm chromatin condensation. The main findings from this study were that addition of GSH to the thawing medium resulted in: (1) a higher motility and progressive motility; (2) a higher number of non-capacitated viable spermatozoa; (3) higher number of viable spermatozoa with intact acrosome; (4) a reduction in ROS generation and (5) lower chromatin condensation. In a second study, the additions of reduced (GSH, 5 mM) or oxidized glutathione (GSSG, 2.5 mM) to the thawing media were evaluated. We confirmed the protective effect of GSH on the sperm functionality. The addition of GSSG to the thawing media was less protective to sperm functions compared to GSH. Addition of GSH to the thawing extender could be of significant benefit in improving the function and fertilizing capacity of frozen goat spermatozoa. The information derived from this study suggests the importance of oxidative stress as responsible for cryo-injury to spermatozoa and opens new windows to explore the practical application of antioxidants to improve the quality of post-thaw goat semen. (C) 2013 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.