Proteomic analysis of two divergently responding potato genotypes (Solanum tuberosum L.) following osmotic stress treatment in vitro
Proteomic analysis of two divergently responding potato genotypes (Solanum tuberosum L.) following osmotic stress treatment in vitro
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DOI：
10.1016/j.jprot.2016.04.048
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发表时间：
2016-06-30
影响因子：
3.3
通讯作者：
Winkelmann, Traud
中科院分区：
文献类型：
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作者：
Buendig, Christin;Jozefowicz, Anna Maria;Winkelmann, Traud
淀粉马铃薯（Solanum tuberosum L）在淀粉、乙醇和生物聚合物的生产中具有重要意义。由于干旱期的预测增加，培育耐旱的淀粉马铃薯是必不可少的。本研究旨在阐明引起耐旱性的生理机制。比较了两种耐旱性不同的基因型。我们施加渗透胁迫，这是一个已知的组成部分，干旱胁迫在体外条件下。在对照培养基和提供有0.2M山梨醇的培养基上培养11天后收获茎尖。采用双向等电聚焦十二烷基硫酸钠聚丙烯酰胺凝胶电泳（2D-IEF/SDS-PAGE）分析其蛋白质组。在679个不同的蛋白质点中，118个和20个具有差异丰度的点分别在敏感和耐受基因型中被发现，在施加胁迫后。使用质谱，在100个差异丰富的点的蛋白质进行了鉴定，这些蛋白质中的大多数来自叶绿体。对于敏感的基因型，蛋白酶抑制剂及其前体的丰度增加，应激反应蛋白的变化和改变的RNA/DNA结合反应进行了观察。差异丰富的斑点的宽容的基因型包括一个伴侣和一个过氧化氢解毒protein.Biological意义：我们的研究结果表明，这两种基因型有不同的反应，渗透胁迫的蛋白质降解和活性氧（ROS）的清除和生产。我们的数据表明，耐受基因型可能会更快地适应施加的压力。比较时间分析可能会提供进一步的见解，这些快速变化，并协助生物标志物的发展。(C)© 2016 Elsevier B. V.版权所有。
Starch potatoes (Solanum tuberosum L) are of interest for production of starch, ethanol, and biopolymers. Due to the predicted increase in drought periods, the breeding of starch potatoes for drought tolerance is essential. This study aims to elucidate the physiological mechanisms that give rise to drought tolerance. Two genotypes contrasting in drought tolerance were compared. We applied osmotic stress which is a known component of drought stress under in vitro conditions. Shoot tips were harvested after 11 days of culture on control medium and medium supplied with 0.2 M sorbitol. Their proteomes were analyzed using two-dimensional isoelectric focussing sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D-IEF/SDS-PAGE). Of a total of 679 distinct protein spots, 118 and 20 spots with differential abundance were found in the sensitive and the tolerant genotype, respectively, after the application of stress. Using mass spectrometry, the proteins in 100 differentially abundant spots were identified; a majority of these proteins were from the chloroplast. For the sensitive genotype, an increase in the abundance of proteinase inhibitors and their precursors, changes in stress responsive proteins and an altered RNA/DNA-binding response were observed. The differentially abundant spots of the tolerant genotype comprised one chaperone and one hydrogen peroxide detoxifying protein.Biological significance: Our findings reveal that the two genotypes have different responses to osmotic stress in terms of protein degradation and reactive oxygen species (ROS) scavenging and production. Our data suggest that the tolerant genotype might adjust to the applied stress more quickly. A comparative temporal analysis might provide further insights into these rapid changes and assist in the development of biomarkers. (C) 2016 Elsevier B.V. All rights reserved.