Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis
Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis
复制标题
京东青蛙皮肤分泌物中两个新的抗菌肽家族
DOI：
10.1016/j.biochi.2011.07.021
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发表时间：
2012-02-01
期刊：
影响因子：
3.9
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
--
作者：
Chen, Zhongming;Yang, Xinwang;Zhang, Yun
新的天然抗菌肽（AMP）的特性可以帮助解决细菌对目前使用的抗生素的严重耐药性问题。本研究分析了景东湍蛙（Amolops jingdongensis）中两个具有多种生物活性的AMP家族。第一个家族的肽，命名为jindongenin-1a，长度为24个氨基酸; jindongenin-1a的BLAST搜索显示与其他AMP没有序列相似性。第二个家族由两个肽组成，每个肽含有29个氨基酸残基。这些肽与palustrin-2的AMP具有高序列相似性，因此被命名为palustrin-2AJ 1和palustrin-2AJ 2。已克隆编码这些AMP的cDNA序列，并通过蛋白质测序确定了每个AMP的推导蛋白质序列。序列和结构分析表明，每个前体是由一个假定的信号肽，N-末端间隔，加工位点和二硫键桥接的七肽段在C-末端。我们合成了金东素-1a和palustrin-AJ1，并对其抗菌、溶血、抗氧化和细胞毒活性进行了研究。这两种肽对标准菌株和临床分离菌株显示出广谱抗菌活性。此外，在我们的实验条件下，它们对人和兔红细胞表现出弱的溶血活性。此外，这些肽还显示出对K562和HT 29哺乳动物细胞系的细胞毒性活性和低抗氧化活性。这些发现提供了有益的见解，将是有用的抗感染肽剂的设计。(C)2011年Elsevier Masson SAS。All rights reserved.
The characterization of new natural antimicrobial peptides (AMPs) can help to solve the serious problem of bacterial resistance to currently used antibiotics. In the current study, we analyzed two families of AMPs from the Chinese torrent frog Amolops jingdongensis with a range of bioactivities. The first family of peptides, named jindongenin-1a, is 24 amino acids in length; a BLAST search of jindongenin-1a revealed no sequence similarity with other AMPs. The second family consists of two peptides containing 29 amino acid residues each. These peptides have high sequence similarity with the AMPs of palustrin-2 and are therefore designated palustrin-2AJ1 and palustrin-2AJ2. The cDNA sequences encoding these AMPs have been cloned and the deduced protein sequence of each AMP has been determined by protein sequencing. Sequence and structural analysis showed that each precursor is composed of a putative signal peptide, an N-terminal spacer, a processing site and a disulfide-bridged heptapeptide segment at the C-terminus. We synthesized jindongenin-1a and palustrin-AJ1 to test their antimicrobial, hemolytic, antioxidative and cytotoxic activities. These two peptides showed broad-spectrum antimicrobial activity to standard and clinically isolated strains of bacteria. In addition, they exhibited weak hemolytic activity to human and rabbit erythrocytes under our experimental conditions. Moreover, these peptides also displayed cytotoxic activity against the K562 and HT29 mammalian cell lines and low anti-oxidant activity. These findings provide helpful insight that will be useful in the design of anti-infective peptide agents. (C) 2011 Elsevier Masson SAS. All rights reserved.