A BTB domain-containing gene is upregulated by immune challenge.
A BTB domain-containing gene is upregulated by immune challenge.
复制标题
DOI：
10.1002/arch.20421
复制
发表时间：
2011-06
影响因子：
2.2
通讯作者：
G. Wang;Peng-Cheng Liu;Jin-Xing Wang;Xiao-Fan Zhao
中科院分区：
文献类型：
--
作者：
G. Wang;Peng-Cheng Liu;Jin-Xing Wang;Xiao-Fan Zhao
20-羟基蜕皮激素（20 E）是一种重要的昆虫生长调节激素。虽然先前的证据显示20 E促进昆虫的先天免疫，但所涉及的机制仍不清楚。本研究克隆了棉铃虫HaBBP基因，该基因包含BTB（broad-complex，tramtrack，bric-a-brac），BACK（BTB和carboxyl-terminal kelch repeats）和PHR（PAM，highwire，RPM）结构域。HaBBP的RT-PCR和western blot分析结果表明，与摄食期相比，蜕皮期和变态期的脂肪体和血细胞中HaBBP的mRNA和蛋白水平均有所升高。HaBBP在血细胞中被20 E上调。在表皮细胞系（HaEpi）中敲低20 E受体EcR-B1和异二聚体伴侣超气门蛋白USP 1阻断HaBBP的转录。HaBBP分布于粒细胞和浆细胞中。大肠杆菌的免疫刺激引起血细胞和脂肪体中HaBBP的上调。因此，HaBBP受20 E信号通路调控，可能参与昆虫的先天免疫。
20-Hydroxyecdysone (20E) is an important hormone that regulates the development of insects. Although previous evidence revealed that 20E promotes innate immunity in insects, the mechanism involved is still unclear. In this study, the HaBBP gene from Helicoverpa armigera is cloned, which contains BTB (broad-complex, tramtrack, and bric-a-brac), a BACK (BTB and carboxyl-terminus kelch repeats), and PHR (PAM, highwire, and RPM) domains. RT-PCR analysis of HaBBP and western blot analysis of HaBBP show that the mRNA and protein level are higher in the fat body and hemocytes during the molting and metamorphic stages compared with the feeding stage. HaBBP was upregulated by 20E in hemocytes. Knockdown of the 20E receptor EcR-B1 and the heterodimeric partner ultraspiracle protein USP1 in an epidermal cell line (HaEpi) blocked the transcription of HaBBP. HaBBP is distributed in granulocytes and plasmatocytes. Immune stimulation by Escherichia coli caused the upregulation of HaBBP in both hemocytes and fat body. Thus, HaBBP is regulated by the 20E signaling pathway, and is likely involved in the insect innate immunity.