Distribution and activation of eosinophils in inflammatory bowel disease using an improved immunohistochemical technique
Distribution and activation of eosinophils in inflammatory bowel disease using an improved immunohistochemical technique
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DOI：
10.1002/path.904
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发表时间：
2001-08-01
影响因子：
7.3
通讯作者：
Woolley, DE
中科院分区：
文献类型：
--
作者：
Jeziorska, M;Haboubi, N;Woolley, DE
嗜酸性粒细胞是炎症性肠病（IBD）的公认特征，但其在克罗恩病（CD）和溃疡性结肠炎（UC）中的组织分布和功能重要性仍不清楚。本研究描述了一种改进的免疫组化协议，以确定嗜酸性粒细胞在IBD（n=40）的全层肠壁标本和他们的原位关系与趋化因子嗜酸性粒细胞趋化因子和RANTES。使用单克隆抗体（EG 1 + EG 2 + MBP）的组合免疫组织化学鉴定嗜酸性粒细胞，这是一种上级于其他方法的超灵敏技术，其组织分布与嗜酸性粒细胞趋化因子、RANTES、肥大细胞和中性粒细胞相关。在CD和UC的活动性、暴发性炎症中均观察到嗜酸性粒细胞数量增加（高达400个细胞/mm 2），这与炎症的严重程度有关，而与这两种疾病的诊断无关。趋化因子eotaxin（CCL 11）和RANTES（CCL 5）在显示嗜酸性粒细胞增多的IBD组织中上调。中性粒细胞和肥大细胞通常与嗜酸性粒细胞蓄积相关。嗜酸性粒细胞数量及其原位活化在活动性IBD中增加，而非慢性IBD。这些观察结果强烈表明，嗜酸性粒细胞及其强效介质在CD和UC的许多病理生理学症状中发挥关键作用，在活动性炎症性肠病中，嗜酸性粒细胞占所有粒细胞的主要比例。版权所有（C）2001约翰威利父子有限公司
Eosinophils are a recognized feature of inflammatory bowel disease (IBD), but their tissue distribution and functional importance in Crohn's disease (CD) and ulcerative colitis (UC) remain obscure. This study describes an improved immunohistochemical protocol to identify eosinophils in full thickness bowel wall specimens of IBD (n=40) and their in situ relationships with the chemoattractants eotaxin and RANTES. Eosinophils were identified using immunohistochemistry with a combination of monoclonal antibodies (EG1 + EG2 + MBP), an ultrasensitive technique superior to other methodologies, and their tissue distributions were related to those for eotaxin, RANTES, mast cells and neutrophils. Increased numbers of eosinophils (up to 400 cells/mm(2)) were observed in active, fulminant inflammation in both CD and UC, this being related to the severity of inflammation and not the diagnosis of the two disorders. The chemoattractants eotaxin (CCL11) and RANTES (CCL5) were upregulated in IBD tissues showing eosinophilia. Neutrophils and mast cells were commonly associated with eosinophil accumulations. Eosinophil numbers and their in situ activation are increased in active rather than chronic IBD. The observations strongly suggest a pivotal role for the eosinophil and its potent mediators in many pathophysiological symptoms of CD and UC, where it represents the major proportion of all granulocytic cells in active inflammatory bowel disease. Copyright (C) 2001 John Wiley & Sons, Ltd.