Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2
Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2
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DOI：
10.1016/j.jbiosc.2012.08.015
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发表时间：
2013-01-01
影响因子：
2.8
通讯作者：
Endo, Ginro
中科院分区：
文献类型：
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作者：
Ikeda-Ohtsubo, Wakako;Miyahara, Morio;Endo, Ginro
在生物强化技术中，接种物在处理系统中的存活是先决条件，但仍然是一个关键的障碍。在这项研究中，我们生物强化的猪场废水处理系统的反硝化池与反硝化细菌施氏假单胞菌菌株TR 2在两个中试规模的实验，以减少一氧化二氮（N2 O），一种气体的环境问题。在实验室中，菌株TR 2生长良好，并在广泛的温度范围（28-40摄氏度）内与高浓度的亚硝酸盐（5-10 mM）一起存活。在中试规模实验的第一次扩大中，接种到反硝化池中的菌株TR 2在条件（30 ℃，类似于0.1mM亚硝酸盐）下仅存活2-5天。相反，在实验室确定的有利于细菌生长的条件（40-45 ℃，2-5 mM亚硝酸盐）下的第二次扩增中，菌株TR 2存活超过32天。在通过实时定量PCR确认菌株TR 2存在期间，即使在反硝化和硝化池中的亚硝酸盐积累条件下，N2 O排放也保持在低水平，这提供了菌株TR 2可以在中试规模系统中减少N2 O的间接证据。我们的研究结果记录了在实验室中确定的有利于菌株TR 2的生长条件的有效应用，以维持该菌株在中试规模反应器系统中的生长和性能，并因此减少N2 O排放。(C)2012年，生物技术学会，日本。All rights reserved.
In bioaugmentation technology, survival of inoculant in the treatment system is prerequisite but remains to be a crucial hurdle. In this study, we bioaugmented the denitrification tank of a piggery wastewater treatment system with the denitrifying bacterium Pseudomonas stutzeri strain TR2 in two pilot-scale experiments, with the aim of reducing nitrous oxide (N2O), a gas of environmental concern. In the laboratory, strain TR2 grew well and survived with high concentrations of nitrite (5-10 mM) at a wide range of temperatures (28-40 degrees C). In the first augmentation of the pilot-scale experiment, strain TR2 inoculated into the denitrification tank with conditions (30 degrees C, similar to 0.1 mM nitrite) survived only 2-5 days. in contrast, in the second augmentation with conditions determined to be favorable for the growth of the bacterium in the laboratory (40-45 degrees C, 2-5 mM nitrite), strain TR2 survived longer than 32 days. During the time when the presence of strain TR2 was confirmed by quantitative real-time PCR, N2O emission was maintained at a low level even under nitrite-accumulating conditions in the denitrification and nitrification tanks, which provided indirect evidence that strain TR2 can reduce N2O in the pilot-scale system. Our results documented the effective application of growth conditions favorable for strain TR2 determined in the laboratory to maintain growth and performance of this strain in the pilot-scale reactor system and the decrease of N2O emission as the consequence. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.