Photodynamic therapy with ATX-S10•Na(II) inhibits synovial sarcoma cell growth
Photodynamic therapy with ATX-S10•Na(II) inhibits synovial sarcoma cell growth
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DOI：
10.1007/s11999-008-0284-6
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发表时间：
2008-07-01
影响因子：
4.2
通讯作者：
Ozaki, Toshifumi
中科院分区：
文献类型：
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作者：
Takeda, Ken;Kunisada, Toshiyuki;Ozaki, Toshifumi
光动力疗法（PDT）是一种有效的癌症治疗方式，其允许选择性破坏恶性肿瘤细胞。我们询问PDT是否可以抑制滑膜肉瘤细胞在体内和体外的生长。我们使用ATX-S10中心点Na（II）和二极管激光分析了滑膜肉瘤细胞系（SYO-1）的PDT。ATX-S10中心点Na（II）的光动力疗法显示对培养的SYO-1细胞的体外细胞毒作用。PDT的体外效应依赖于ATX-S10中心点Na（II）的处理浓度和激光照射剂量。在静脉注射SYO-1后6小时内，从携带SYO-1的裸鼠切除的肿瘤组织标本中检测到ATX-S10中心点Na（II），但在注射后12小时，其从肿瘤中消除。光动力学疗法抑制荷SYO-1裸鼠的肿瘤生长，高剂量照射在组织学标本中未诱导活的肿瘤细胞。携带SYO-1的裸鼠肿瘤边缘切除后进行的光动力疗法降低了肿瘤局部复发率。我们的研究结果表明，使用ATX-S10中心点Na（II）和激光照射的PDT可能是滑膜肉瘤的一种潜在有用的治疗方法，特别是减少手术切缘和保留邻近肿瘤的重要解剖结构。
Photodynamic therapy (PDT) is an effective cancer treatment modality that allows selective destruction of malignant tumor cells. We asked whether PDT could inhibit in vivo and in vitro growth of synovial sarcoma cells. We analyzed PDT using ATX-S10 center dot Na(II) and a diode laser for a synovial sarcoma cell line (SYO-1). Photodynamic therapy with ATX-S10 center dot Na(II) showed an in vitro cytotoxic effect on the cultured SYO-1 cells. The in vitro effect of PDT depended on the treatment concentration of ATX-S10 center dot Na(II) and the laser dose of irradiation. ATX-S10 center dot Na(II) was detected in the tumor tissue specimens that were excised from nude mice bearing SYO-1 within 6 hours after intravenous injection, but it was eliminated from the tumor 12 hours after injection. Photodynamic therapy suppressed the tumor growth of nude mice bearing SYO-1, and high-dose irradiation induced no viable tumor cells in histologic specimens. Photodynamic therapy performed after marginal resection of the tumor of nude mice bearing SYO-1 reduced the rate of local recurrence of the tumor. Our results suggest PDT using ATX-S10 center dot Na(II) and laser irradiation may be a potentially useful treatment for synovial sarcoma, especially to reduce the surgical margin and preserve critical anatomic structures adjacent to the tumor.