Distribution of alginate oligomers in Catharanthus roseus and Wasabia japonica cells cultured in a medium containing alginate oligomers
Distribution of alginate oligomers in Catharanthus roseus and Wasabia japonica cells cultured in a medium containing alginate oligomers
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含有藻酸盐低聚物的培养基中培养的长春花和山葵细胞中藻酸盐低聚物的分布
DOI：
10.1023/a:1016087222434
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发表时间：
2002
影响因子：
2.7
通讯作者：
Hideo Tanaka
中科院分区：
文献类型：
--
作者：
H. Aoyagi;J. Yasuhira;A. Kuno;K. Ono;Chiharu Akimoto;S. Yoshida;Hideo Tanaka
采用7-氨基-1,3-萘二磺酸单钾偶联的方法，研究了海藻酸寡聚物（AO）在植物培养细胞中的分布。将AO-ANDS以0.5 g l−1的浓度加入到花楸细胞培养液中，荧光显微镜观察到AO-ANDS粘附在细胞上。在红桃原生质体中，不仅在细胞壁中，而且在细胞膜和细胞质中都发现了ao - and。当玫瑰花在含有低聚半乳糖醛酸的培养基中培养时，作为内源性激发子，在玫瑰花细胞的细胞壁、细胞膜和细胞质中也发现了这种现象。在Wasabia japonica细胞中也获得了类似的结果。
Distribution of alginate oligomers (AO) which are endogenous elicitor-like substances, in cultured plant cells were investigated by using AO conjugated with monopotassium 7-amino-1,3-naphthalenedisulfonate (ANDS). When AO-ANDS was added at 0.5 g l−1 to the Catharanthus roseus cell culture, it adhered to the cells as observed by fluorescence microscopy. Using protoplasts of C. roseus, AO-ANDS was found not only in the cell walls but also in the cell membrane and cytoplasm. When C. roseus was cultivated in a medium containing oligo-galacturonic acids, as an endogenous elicitor, this was also found in the cell wall, cell membrane and cytoplasm of C. roseus cells. Similar results were also obtained with Wasabia japonica cells.