Characterization of the Ubiquitin Specific Protease (USP) family members in the fast and slow muscle fibers from Chinese perch (Siniperca chuatsi).
Characterization of the Ubiquitin Specific Protease (USP) family members in the fast and slow muscle fibers from Chinese perch (Siniperca chuatsi).
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DOI：
10.1016/j.gene.2018.07.013
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发表时间：
2018-11
期刊：
影响因子：
3.5
通讯作者：
Fangliang Zhang;Yulong Li;Lin Chen;Jia Cheng;Ping Wu;W. Chu;Jianshe Zhang
中科院分区：
文献类型：
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作者：
Fangliang Zhang;Yulong Li;Lin Chen;Jia Cheng;Ping Wu;W. Chu;Jianshe Zhang
骨骼肌萎缩是由禁食、废用和其他系统性疾病引起的。肌肉萎缩与通过泛素蛋白酶体系统（UPS）增加的肌肉蛋白降解有关。泛素特异性蛋白酶（USP），也称为去泛素化酶，调节骨骼肌中多种细胞过程。在我们的研究中，在鲈鱼骨骼肌转录组中鉴定的41个USP家族成员中，有24个USP在快肌和慢肌纤维中差异表达。研究了4种肌肉相关USP（USP 10、USP 14、USP 19、USP 45）在快肌和慢肌中响应禁食4天和7天的表达谱。结果表明，禁食4 d和7 d后，大鼠快肌中USP 10、USP 14和USP 19的表达显著增加。但只有USP 10和USP 14在禁食7天时在慢肌中显著增加。MAFbx和MuRF1的表达上调，而主要肌原纤维基因的表达下调，表明这四个USP都参与了快肌和慢肌的蛋白质降解。
Skeletal muscle atrophy results from fasting, disuse and other systemic diseases. Muscle atrophy is associated with increased muscle protein degradation via the Ubiquitin proteasome system (UPS). The Ubiquitin Specific Proteases (USPs), also known as deubiquitinating enzymes, regulates a wide variety of cellular processes in skeletal muscle. In our study, among the 41 members of the USP family identified in the skeletal muscle transcriptome of Chinese perch, 24 USPs were differentially expressed between the fast and slow muscle fibers. The expressional profile of 4 muscle-related USPs (USP10, USP14, USP19, USP45) was investigated in the fast and slow muscle in response to fasting at 4 and 7 days. The results showed that the expression of USP10, USP14 and USP19 was significantly increased in the fast muscle after fasting for 4 days and 7 days. But only the USP10 and USP14 had significantly increased at 7 days of fasting in the slow muscle. The expression of MAFbx and MuRF1 up-regulated and major myofibrillar genes down-regulated, indicating that all of these four USPs are involved in the protein degradation of the fast and slow muscle.