Molecular characterization of newly recognized rodent parvoviruses.
Molecular characterization of newly recognized rodent parvoviruses.
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新识别的啮齿动物细小病毒的分子特征。
DOI：
10.1099/0022-1317-77-5-899
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Riley,LK
中科院分区：
文献类型：
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作者：
Besselsen,DG;Pintel,DJ;Purdy,GA;Besch-Williford,CL;Franklin,CL;HookJr,RR;Riley,LK
已分离出几种与原型啮齿动物细小病毒不同的自主啮齿动物细小病毒。这些包括小鼠细小病毒（MPV）的变种、被命名为仓鼠细小病毒（HaPV）的仓鼠分离株以及被命名为MVM-Cutter或MVM（c）的小鼠微小病毒（MVM）的变种株。在这项研究中，确定了这些新分离株的病毒基因组编码区的 DNA 序列和预测的蛋白质序列，并与 MVM [MVM(i) 和 MVM(p)] 的免疫抑制株和原型株、啮齿动物细小病毒 H-1 和 LuIII（一种宿主来源不确定的自主细小病毒）进行比较。序列比较显示MPV分离株几乎相同，HaPV与MPV非常相似，MVM(c)与MVM(i)和MVM(p)最相似。血凝抑制测定表明，MPV 和 HaPV 代表与先前表征的啮齿动物细小病毒血清型不同的两种血清型，而 MVM(c) 属于 MVM 血清型。每种新分离的啮齿动物细小病毒都包裹着主要为负义的 5 kb DNA 基因组，并编码两种非结构蛋白（NS1 和 NS2）和两种结构病毒蛋白（VP1 和 VP2）。这些研究表明 MPV 和 HaPV 是自主细小病毒，与之前表征的细小病毒不同，并且 MVM(c) 是 MVM 的变种毒株，与 MVM(i) 和 MVM(p) 不同。
Several autonomous rodent parvoviruses distinct from the prototypic rodent parvoviruses have been isolated. These include variants of a mouse parvovirus (MPV), a hamster isolate designated hamster parvovirus (HaPV), and a variant strain of minute virus of mice (MVM) designated MVM-Cutter or MVM(c). In this study, the DNA sequence of the coding regions of the viral genome and the predicted protein sequences for each of these new isolates were determined and compared to the immunosuppressive and prototypic strains of MVM [MVM(i) and MVM(p)], the rodent parvovirus H-1, and LuIII, an autonomous parvovirus of uncertain host origin. Sequence comparisons showed that the MPV isolates were almost identical, HaPV was very similar to MPV, and MVM(c) was most similar to MVM(i) and MVM(p). Haemagglutination inhibition assays revealed that MPV and HaPV represent two serotypes distinct from previously characterized rodent parvovirus serotypes while MVM(c) belongs to the MVM serotype. Each of the newly isolated rodent parvoviruses was shown to encapsidate a predominantly negative-sense 5 kb DNA genome and to encode two nonstructural proteins (NS1 and NS2) and two structural viral proteins (VP1 and VP2). These studies indicate that MPV and HaPV are autonomous parvoviruses distinct from previously characterized parvoviruses and MVM(c) is a variant strain of MVM distinct from MVM(i) and MVM(p).