Constituents of the anti-asthma herbal formula ASHMI(TM) synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells, and eotaxin by human lung fibroblasts in vitro.
Constituents of the anti-asthma herbal formula ASHMI(TM) synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells, and eotaxin by human lung fibroblasts in vitro.
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DOI：
10.3736/jintegrmed2013029
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Li XM
中科院分区：
文献类型：
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作者：
Jayaprakasam B;Yang N;Wen MC;Wang R;Goldfarb J;Sampson H;Li XM
抗哮喘草药干预(ASHMI™)是我们实验室开发的三种传统中草药的组合，已在过敏性哮喘小鼠模型和哮喘患者的双盲安慰剂对照临床试验中证明有效。这项研究旨在确定阿什米™的各个草本成分的抗炎作用是否具有协同作用。采用酶联免疫吸附试验检测灵芝、苦参、甘草水提物对小鼠记忆Th2细胞(D10.G4.1)分泌Th2细胞因子和人肺成纤维细胞(HLF-1)分泌嗜酸性粒细胞趋化因子-1的影响。根据浓度-效应曲线参数计算相互作用指数，确定潜在的协同效应。灵芝、苦参、甘草单味提取物和阿司米(单味提取物组合)可抑制小鼠记忆Th2细胞产生IL-4和IL-5，抑制HLF-1细胞产生嗜酸性粒细胞趋化因子-1。阿施米、灵芝、苦参和甘草对IL-4产生的平均25%抑制浓度(IC_(25))分别为30.9、79.4、123和64.6，对IL-5的产生分别为30.2、2 63、123.2和10 0，对嗜酸性粒细胞趋化因子-1的平均抑制浓度分别为13.2、16.2、30.2和2 5.1。阿司米、灵芝、苦参、甘草对IL-4产生的IC50值分别为158.5、239.9、446.7和281.8，对嗜酸性粒细胞趋化因子-1的IC50值分别为38.1%、33.1%、100%和158.5。在IC25，阿什米成分的相互作用指数为：IL-4为0.35，IL-5为0.21，嗜酸性粒细胞趋化蛋白-1为0.59。IC50值时的相互作用指数分别为：IL-4为0.50，嗜酸性粒细胞趋化因子-1抑制为0.62。对IL-5的抑制作用未达到IC50。所有相互作用指数均小于1，表明存在协同作用。通过相互作用指数值的比较，我们发现Ashmi™中的成分协同抑制嗜酸性粒细胞趋化因子-1的产生以及Th2型细胞因子的产生。
Anti-asthma herbal medicine intervention (ASHMI™), a combination of three traditional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMI™ exhibited synergy. Effects of ASHMI and its components aqueous extracts of Lingzhi (Ganoderma lucidum), Kushen (Sophora flavescens) and Gancao (Glycyrrhiza uralensis), on Th2 cytokine secretion by murine memory Th2 cells (D10.G4.1) and eotaxin-1 secretion by human lung fibroblast (HLF-1) cells were determined by measuring levels in culture supernatants by enzyme-linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters. Individual Lingzhi, Kushen and Gancao extracts and ASHMI (the combination of individual extracts) inhibited production of interleukin (IL)-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration (IC25) values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50 values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-1. The interaction indices at IC50 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC50 values. All interaction indices were below 1 which indicated synergy. By comparing the interaction index values, we find that constituents in ASHMI™ synergistically inhibited eotaxin-1 production as well as Th2 cytokine production.