Coptis extracts enhance the anticancer effect of estrogen receptor antagonists on human breast cancer cells.
Coptis extracts enhance the anticancer effect of estrogen receptor antagonists on human breast cancer cells.
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DOI：
10.1016/j.bbrc.2008.10.169
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发表时间：
2009-01-09
影响因子：
3.1
通讯作者：
Kang, Jing X.
中科院分区：
文献类型：
--
作者：
Liu, Jing;He, Chengwei;Zhou, Keyuan;Wang, Jingdong;Kang, Jing X.
雌激素受体（ER）拮抗剂已广泛应用于乳腺癌的治疗，但其疗效和耐药性仍是临床关注的问题。本研究的目的是确定黄连，一种抗炎草药的提取物，是否提高ER拮抗剂的抗癌功效。结果表明，ER拮抗剂与黄连粗提物或其纯化化合物小檗碱联合作用对MCF-7细胞（ER+）有协同生长抑制作用，但对MDA-MB-231细胞（ER-）无协同生长抑制作用。在芳香化酶拮抗剂氟维司群的联合治疗中观察到类似的结果。乳腺癌相关基因的表达分析表明，小檗碱能显著下调EGFR、HER 2、bcl-2和考克斯-2的表达，而显著上调IFN-β和p21的表达。我们的研究结果表明，黄连提取物可能是有希望的辅助ER拮抗剂在ER阳性乳腺癌治疗中通过调节多个基因的表达。
Estrogen receptor (ER) antagonists have been widely used for breast cancer treatment, but the efficacy and drug resistance remain to be clinical concerns. The purpose of this study was to determine whether the extracts of coptis, an anti-inflammatory herb, improve the anticancer efficacy of ER antagonists. The results showed that the combined treatment of ER antagonists and the crude extract of coptis or its purified compound berberine conferred synergistic growth inhibitory effect on MCF-7 cells (ER+), but not on MDA-MB-231 cells (ER-). The similar results were observed in the combined treatment of fulvestrant, a specific aromatase antagonist. Analysis of the expression of breast cancer related genes indicated that EGFR, HER2, bcl-2 and COX-2 were significantly downregulated, while IFN-β and p21 were remarkably upregulated by berberine. Our results suggest that coptis extracts could be promising adjuvant to ER antagonists in ER positive breast cancer treatment through regulating expression of multiple genes.
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影响因子：
10.3
作者：
Shou, J;Massarweh, S;Schiff, R
通讯作者：
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45.3
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作者：
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