Chemoproteomic mapping of human milk oligosaccharide (HMO) interactions in cells.
Chemoproteomic mapping of human milk oligosaccharide (HMO) interactions in cells.
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DOI：
10.1039/d2cb00176d
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发表时间：
2022-11-30
影响因子：
4.1
通讯作者：
Huang, Mia L.
中科院分区：
文献类型：
--
作者：
Hassan, Abdullah A.;Wozniak, Jacob M.;Vilen, Zak;Li, Weichao;Jadhav, Appaso;Parker, Christopher G.;Huang, Mia L.
人乳低聚糖(HMOS)是人乳汁中发现的一类非结合的可溶性多糖，具有多种生物活性。虽然最近的研究已经发现了HMOS的许多生物学功能(抗菌、抗炎和益生菌特性)，但参与这些过程的受体和蛋白质结合伙伴还没有得到很好的表征。这在很大程度上可以归因于可溶性糖-蛋白质相互作用的低亲和力和瞬时性质，排除了使用传统的表征技术来调查活细胞中的结合伙伴。在这里，我们介绍了使用合成的可光激活的HMO探针来捕获、丰富和鉴定活细胞中的HMO蛋白靶标，使用基于质谱学的化学蛋白质组学。在使用纯化的凝集素进行初步验证研究后，我们在活的小鼠巨噬细胞中描绘了HMO探针的靶标。使用这一策略，我们在多个细胞隔间绘制了数百个HMO结合伙伴的图谱，包括许多已知的糖结合蛋白以及许多以前未知的结合多糖的蛋白质。我们希望我们的发现将为未来关于HMOS如何调节蛋白质功能的不同角色的研究提供信息。人乳低聚糖(HMOS)是人乳汁中发现的一类非结合的可溶性多糖，具有多种生物活性。
Human milk oligosaccharides (HMOs) are a family of unconjugated soluble glycans found in human breast milk that exhibit a myriad of biological activity. While recent studies have uncovered numerous biological functions for HMOs (antimicrobial, anti-inflammatory & probiotic properties), the receptors and protein binding partners involved in these processes are not well characterized. This can be attributed largely in part to the low affinity and transient nature of soluble glycan–protein interactions, precluding the use of traditional characterization techniques to survey binding partners in live cells. Here, we present the use of synthetic photoactivatable HMO probes to capture, enrich and identify HMO protein targets in live cells using mass spectrometry-based chemoproteomics. Following initial validation studies using purified lectins, we profiled the targets of HMO probes in live mouse macrophages. Using this strategy, we mapped hundreds of HMO binding partners across multiple cellular compartments, including many known glycan-binding proteins as well as numerous proteins previously not known to bind glycans. We expect our findings to inform future investigations of the diverse roles of how HMOs may regulate protein function. Human milk oligosaccharides (HMOs) are a family of unconjugated soluble glycans found in human breast milk that exhibit a myriad of biological activity.
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影响因子：
4
作者：
Bojar, Daniel;Meche, Lawrence;Meng, Guanmin;Eng, William;Smith, David F.;Cummings, Richard D.;Mahal, Lara K.
通讯作者：
Mahal, Lara K.
影响因子：
3.9
作者：
Ge, Sha-Sha;Chen, Biao;Wu, Yuan-Yuan;Long, Qing-Su;Zhao, Yong-Liang;Wang, Pei-Yi;Yang, Song
通讯作者：
Yang, Song
影响因子：
5.8
作者：
Di Carluccio C;Forgione RE;Montefiori M;Civera M;Sattin S;Smaldone G;Fukase K;Manabe Y;Crocker PR;Molinaro A;Marchetti R;Silipo A
通讯作者：
Silipo A
影响因子：
3
作者：
Haudek, Kevin C.;Spronk, Kimberly J.;Voss, Patricia G.;Patterson, Ronald J.;Wang, John L.;Arnoys, Eric J.
通讯作者：
Arnoys, Eric J.
影响因子：
3.1
作者：
LIKHOSHERSTOV, LM;NOVIKOVA, OS;KOCHETKOV, NK
通讯作者：
KOCHETKOV, NK