Bis(8-anilinonaphthalene-1-sulfonate) as a probe for tubulin decay.
Bis(8-anilinonaphthalene-1-sulfonate) as a probe for tubulin decay.
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双(8-苯胺萘-1-磺酸盐)作为微管蛋白衰变的探针。
DOI：
10.1021/bi00351a035
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Horowitz,PM
中科院分区：
文献类型：
--
作者：
Prasad,AR;Luduena,RF;Horowitz,PM
材料和方法材料Bis-ANS从Molecular Probes， Inc.（Junction City， OR）获得。秋水仙碱和GTP来自Sigma Chemical Co.（St.）。路易,密苏里州)。足臼毒素来自Aldrich Chemical Co.（Milwaukee， WI）。硫酸长春碱是礼来公司（印第安纳州印第安纳波利斯）赠送的一份厚礼。美坦辛（NSC 153858）是美国国家癌症研究所化学疗法发展治疗项目的马修·萨福尼斯博士的礼物。其他试剂均为分析级试剂。方法制备微管蛋白。牛脑从Roegelein公司获得。从牛脑的大脑中制备微管蛋白，并通过磷纤维素层析分解成微管蛋白（Whatman PI 1）。（fellow et al., 1977）。用r （0.15 mg/mL）和map2 （0.3 mg/mL）重组微管蛋白后，通过样品聚合成微管的能力来检测纯化微管蛋白的完整性。浊度测量在350纳米
Materials and MethodsMaterials Bis-ANS was obtained from Molecular Probes, Inc.(Junction City, OR). Colchicine and GTP were from Sigma Chemical Co.(St. Louis, MO). Podophyllotoxin was from Aldrich Chemical Co.(Milwaukee, WI). Vinblastine sulfate was a kind gift from the Eli Lilly Co.(Indianapolis, IN). Maytansine (NSC 153858) was the kind gift of Dr. Matthew Suffness of the Developmental Therapeutics Program, Chemotherapy, National Cancer Institute. All other reagents were of analytical grade.Methods Tubulin Preparation. Cow brains were obtained locally from the Roegelein Co. Microtubule protein was prepared from the cerebrums of cow brains and resolved into tubulin by chromatography on phosphocellulose (Whatman PI 1).(Fellous et al., 1977). The integrity of the purified tubulin was assayed by the ability of a sample of it to polymerizeinto microtubules when reconstituted with r (0.15 mg/mL) and MAP 2 (0.3 mg/mL). Turbidity measurements at 350 nm