THE TRANSCRIPTION TERMINATION SITE AT THE END OF THE EARLY REGION OF BACTERIOPHAGE-T7 DNA
THE TRANSCRIPTION TERMINATION SITE AT THE END OF THE EARLY REGION OF BACTERIOPHAGE-T7 DNA
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DOI：
10.1093/nar/8.10.2119
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
STUDIER, FW
中科院分区：
文献类型：
--
作者：
DUNN, JJ;STUDIER, FW
Eacherichia coliRNA聚合酶在T7DNA早期区域末端的转录终止位点的核苷酸序列已经确定。RNA链终止于DNA序列中的相邻残基：约2/3的链以C结尾，1/3以G结尾。潜在的茎和环结构，包含一个由8个不间断碱基对组成的茎和一个4碱基环，位于链末端前17-18个碱基的中心。抄写再见。ColiRNA聚合酶在体外有效地终止于该位点，但T7RNA聚合酶的转录基本上不受影响。在转录终止位点附近没有RNaseIII的主要切割位点。终止位点位于T7DNA的HpaI片段Q中，其全长446个核苷酸的长序列被测定。其他限制性内切酶的切割位点被方便地定位，以便在终止位点周围操纵DNA序列。确定了T7DNA连接酶蛋白最后82个氨基酸的编码序列，以及终止位点以外可能的晚期T7蛋白的编码序列的开始。
The nucleotide sequence through the transcription termination site forEacherichia coliRNA polymerase at the end of the early region of T7 DNA has been determined. RNA chains terminate at adjacent residues in the DNA sequence: about 2/3 of the chains end in C and 1/3 in G. A potential stem and loop structure, containing a stem of eight uninterrupted base pairs and a four-base loop, is centered 17–18 bases ahead of the chain termini. Transcription byE. coliRNA polymerase terminates efficiently at this sitein vivoandin vitrobut transcription by T7 RNA polymerase is essentially unaffected. There are no primary cleavage sites for RNase III near the transcription termination site. The site of termination lies withinHpaIfragment Q of T7 DNA, whose entire 446-nucleotide long sequence was determined. Cleavage sites for other restriction endonucleases are located conveniently for manipulating the DNA sequence around the termination site. The coding sequence for the last 82 amino acids of the T7 DNA ligase protein was identified, as was the beginning of a coding sequence for a possible late T7 protein from beyond the termination site.