MYB30 transcription factor regulates oxidative and heat stress responses through ANNEXIN-mediated cytosolic calcium signaling in Arabidopsis
MYB30 transcription factor regulates oxidative and heat stress responses through ANNEXIN-mediated cytosolic calcium signaling in Arabidopsis
复制标题
拟南芥中 MYB30 转录因子通过 ANNEXIN 介导的胞质钙信号调节氧化和热应激反应
DOI：
10.1111/nph.14679
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发表时间：
2017-10-01
期刊：
影响因子：
9.4
通讯作者：
Guo, Yan
中科院分区：
文献类型：
--
作者：
Liao, Chancan;Zheng, Yuan;Guo, Yan
胞浆钙信号是植物在逆境条件下调节下游反应的关键。在拟南芥胁迫诱导胞浆游离钙缺陷突变体的遗传筛选中，（[Ca 2 +]（cyt））升高，我们鉴定了R2 R3-MYB转录因子MYB 30作为[Ca 2 +]（cyt）的调节因子，缺乏MYB 30蛋白的植株对氧化和热胁迫的响应表现出更大的[Ca 2 +]（cyt）升高。实时逆转录-聚合酶链反应（RT-PCR）结果表明，一些ANNEXIN（ANN）基因的表达，编码钙调节膜结合蛋白调节胞浆钙的签名，上调myb 30突变体。进一步分析表明MYB 30与ANN 1和ANN 4的启动子结合并抑制其表达。myb 30突变体对甲基紫精（MV）和热胁迫敏感。H_2O_2和热诱导myb 30细胞胞浆内[Ca ~（2+）]（cyt）的异常依赖于ANN蛋白的功能。此外，在缺乏ANN功能的突变体或应用LaCl 3，钙通道阻滞剂，myb 30的MV和热敏感性被抑制。这些结果表明，MYB 30通过钙信号调节氧化和热应激反应，这至少部分是由ANN 1和ANN 4介导的。
Cytosolic calcium signaling is critical for regulating downstream responses in plants encountering unfavorable environmental conditions.In a genetic screen for Arabidopsis thaliana mutants defective in stress-induced cytosolic free Ca2+ ([Ca2+](cyt)) elevations, we identified the R2R3-MYB transcription factor MYB30 as a regulator of [Ca2+](cyt) in response to H2O2 and heat stresses.Plants lacking MYB30 protein exhibited greater elevation of [Ca2+](cyt) in response to oxidative and heat stimuli. Real-time reverse transcription-polymerase chain reaction (RT-PCR) results indicated that the expression of a number of ANNEXIN (ANN) genes, which encode Ca2+-regulated membrane-binding proteins modulating cytosolic calcium signatures, were upregulated in myb30 mutants. Further analysis showed that MYB30 bound to the promoters of ANN1 and ANN4 and repressed their expression. myb30 mutants were sensitive to methyl viologen (MV) and heat stresses. The H2O2- and heat-induced abnormal [Ca2+](cyt) in myb30 was dependent on the function of ANN proteins. Moreover, the MV and heat sensitivity of myb30 was suppressed in mutants lacking ANN function or by application of LaCl3, a calcium channel blocker.These results indicate that MYB30 regulates oxidative and heat stress responses through calcium signaling, which is at least partially mediated by ANN1 and ANN4.