A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila.
A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila.
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DOI：
10.1016/j.xpro.2022.101878
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发表时间：
2023-03-17
期刊：
影响因子：
--
通讯作者：
Kumar, Justin P.
中科院分区：
文献类型：
--
作者：
Weasner, Brandon P.;Brown, Haley E.;Policastro, Robert;Weasner, Bonnie M.;Kumar, Justin P.
使用核酸酶（CUT&RUN）测序进行靶下切割和释放是一种用于研究基因调控的技术。本文提出的方案已成功用于鉴定果蝇眼触角盘基因组内组蛋白修饰的模式。以其目前的形式，它可以用于分析其他成虫盘的基因组特征。它可以被修饰以用于其他组织和应用，包括鉴定转录因子占据的模式。基于CUT& RUN的组蛋白修饰图谱优化用于果蝇成虫盘蛋白A/G-MNase的纯化用于CUT&RUN Pipeline对CUT&RUN测序数据进行生物信息学分析出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和道德准则。靶下切割和核酸酶释放（CUT&RUN）测序是一种用于研究基因调控的技术。这里提出的协议已成功地用于识别果蝇的眼睛触角盘的基因组内的组蛋白修饰的模式。以其目前的形式，它可以用于分析其他成虫盘的基因组特征。它可以被修饰以用于其他组织和应用，包括鉴定转录因子占据的模式。
Cleavage Under Targets & Release Using Nucleases (CUT&RUN) sequencing is a technique used to study gene regulation. The protocol presented here has been used successfully to identify the pattern of histone modifications within the genome of the eye-antennal disc of the fruit fly, Drosophila melanogaster. In its present form, it can be used to analyze genomic features of other imaginal discs. It can be modified for use with other tissues and applications including identifying the pattern of transcription factor occupancy. CUT&RUN-based mapping of histone modifications Optimized for use with imaginal discs from Drosophila melanogaster Purification of Protein A/G-MNase for use with CUT&RUN Pipeline for bioinformatic analysis of CUT&RUN sequencing data Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cleavage Under Targets & Release Using Nucleases (CUT&RUN) sequencing is a technique used to study gene regulation. The protocol presented here has been used successfully to identify the pattern of histone modifications within the genome of the eye-antennal disc of the fruit fly, Drosophila melanogaster. In its present form, it can be used to analyze genomic features of other imaginal discs. It can be modified for use with other tissues and applications including identifying the pattern of transcription factor occupancy.
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DOI：
10.1093/bioinformatics/btq033
发表时间：
2010-03-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Quinlan AR;Hall IM
通讯作者：
Hall IM
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.4
作者：
Shen L;Shao N;Liu X;Nestler E
通讯作者：
Nestler E
影响因子：
1.2
作者：
Spratford, Carrie M.;Kumar, Justin P.
通讯作者：
Kumar, Justin P.
影响因子：
5.8
作者：
Lawrence, Michael;Gentleman, Robert;Carey, Vincent
通讯作者：
Carey, Vincent