Imaging and Spectral Characteristics of Amyloid Plaque Autofluorescence in Brain Slices from the APP/PS1 Mouse Model of Alzheimer's Disease
Imaging and Spectral Characteristics of Amyloid Plaque Autofluorescence in Brain Slices from the APP/PS1 Mouse Model of Alzheimer's Disease
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DOI：
10.1007/s12264-019-00393-6
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发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Xu, Fuqiang
中科院分区：
文献类型：
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作者：
Gao, Yunling;Liu, Qing;Xu, Fuqiang
淀粉样蛋白沉积是阿尔茨海默病（AD）的标志性病理损害之一。可通过硫磺素-S、银浸渍、刚果红染色和免疫组织化学反应观察。然而，淀粉样蛋白沉积物产生蓝色自发荧光（auto-F）已被忽略。在这里，我们报告，可见光诱导的老年斑（SP）的自体F检测和验证与传统的方法。将来自APP/PS1（淀粉样前体蛋白/早老素1）转基因小鼠的脑切片固定在载玻片上，冲洗，盖上盖玻片，并观察SP的自体F的成像和光谱特征的细节。然后用上述经典方法处理切片进行比较验证。我们发现，SP auto-F在具有特定发射光谱的蓝-紫激发下最大，并且比经典方法更容易，更灵敏，更可靠。因为它不损伤切片，所以auto-F的观察可以与切片中的所有后染色技术结合，并用于AD的全脑成像。
Amyloid deposits are one of the hallmark pathological lesions of Alzheimer's disease (AD). They can be visualized by thioflavin-S, silver impregnation, Congo red staining, and immunohistochemical reactions. However, that amyloid deposits generate blue autofluorescence (auto-F) has been ignored. Here, we report that visible light-induced auto-F of senile plaques (SPs) was detected and validated with conventional methods. Brain slices from APP/PS1 (amyloid precursor protein/presenilin 1) transgenic mice were mounted on slides, rinsed, coverslipped and observed for details of the imaging and spectral characteristics of the auto-F of SPs. Then the slices were treated with the above classic methods for comparative validation. We found that the SP auto-F was greatest under blue-violet excitation with a specific emission spectrum, and was much easier, more sensitive, and reliable than the classic methods. Because it does not damage slices, observation of auto-F can be combined with all post-staining techniques in slices and for brain-wide imaging in AD.