The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes
The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes
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食线虫真菌的两种角质层降解蛋白酶的晶体结构及其对线虫感染的贡献
DOI：
10.1096/fj.09-136408
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发表时间：
2010-05-01
期刊：
影响因子：
4.8
通讯作者：
Zhang, Ke-Qin
中科院分区：
文献类型：
--
作者：
Liang, Lianming;Meng, Zhaohui;Zhang, Ke-Qin
角质降解蛋白酶参与线虫或昆虫的角质层/蛋壳的分解，角质层/蛋壳是抵抗真菌感染的坚硬物理屏障。了解这些蛋白质的三维结构可以为提高这些真菌在生物防治应用中的有效性提供关键信息。例如，在一个实施例中，通过靶向蛋白质工程。然而，这些蛋白酶的结构仍然未知。在这里，我们报告的结构角质降解蛋白酶从两种食草真菌。这两种结构用X射线晶体学解析，分辨率分别为1.65埃（Ver 112）和2.1埃（PL 646）。PL 646和Ver 112的晶体结构非常相似，并且与另一种真菌白小麦的蛋白酶K的晶体结构相似。在底物结合位点（S1和S4）的残基之间发现结构之间的差异。实验研究表明，不同的酶的水解活性合成肽底物。我们的疏水/亲水和静电特性的分析表明，这两种蛋白质的表面可能发挥重要作用，在真菌感染线虫。这两种晶体结构为研究角质层降解蛋白酶的结构与功能之间的关系提供了坚实的基础。梁，L.，卢，Z.，叶，F.，杨杰，Liu，S.，Sun，Y.，Guo，Y.，中国科学院，Mi，Q.，黄，X.，Zou，C.，中国科学院，孟志，字，Rao，Z.，张，K.- Q.两种食虫真菌表皮降解蛋白酶的晶体结构及其对线虫感染的影响。FASEB J.24，1391-1400（2010）。www.fasebj.org
Cuticle-degrading proteases are involved in the breakdown of cuticle/eggshells of nematodes or insects, a hard physical barrier against fungal infections. Understanding the 3-dimensional structures of these proteins can provide crucial information for improving the effectiveness of these fungi in biocontrol applications, e. g., by targeted protein engineering. However, the structures of these proteases remain unknown. Here, we report the structures of two cuticle-degrading proteases from two species of nematophagous fungi. The two structures were solved with X-ray crystallography to resolutions of 1.65 angstrom (Ver112) and 2.1 angstrom (PL646), respectively. Crystal structures of PL646 and Ver112 were found to be very similar to each other, and similar to that of proteinase K from another fungus Tritirachium album. Differences between the structures were found among residues of the substrate binding sites (S1 and S4). Experimental studies showed that the enzymes differed in hydrolytic activity to synthetic peptide substrates. Our analyses of the hydrophobic/hydrophilic and electrostatic features of these two proteins suggest that their surfaces likely play important roles during fungal infection against nematodes. The two crystal structures provide a solid basis for investigating the relationship between structure and function of cuticle-degrading proteases.-Liang, L., Lou, Z., Ye, F., Yang, J., Liu, S., Sun, Y., Guo, Y., Mi, Q., Huang, X., Zou, C., Meng, Z., Rao, Z., Zhang, K.-Q. The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes. FASEB J. 24, 1391-1400 (2010). www.fasebj.org