ASSESSMENT OF FIBROSIS IN INFARCTED HUMAN HEARTS BY ANALYSIS OF ULTRASONIC BACKSCATTER
ASSESSMENT OF FIBROSIS IN INFARCTED HUMAN HEARTS BY ANALYSIS OF ULTRASONIC BACKSCATTER
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DOI：
10.1161/01.cir.71.4.740
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发表时间：
1985-01-01
期刊：
影响因子：
37.8
通讯作者：
CONYERS, D
中科院分区：
文献类型：
--
作者：
HOYT, RH;COLLINS, SM;CONYERS, D
在动物心脏中，超声背向散射积分的幅度在纤维化心肌中增加。对10例陈旧性心肌梗死切除心脏的超声背向散射与胶原沉积的关系进行了定量研究。将2.25 MHz、50%分数带宽的换能器放置在每个标本的换能器距心外膜的焦点距离处。射频后向散射信号被数字化、平方和积分，以产生积分的超声波后向散射，该后向散射是指来自水/钢界面的后向散射。然后切除被询问的心肌并将其分成2部分。其中一部分被检测了羟脯氨酸，这是胶原蛋白的标志物。第二部分切片，马森S三色染色，并使用计算机辅助图像分析系统进行研究。背向散射积分的大小与羟脯氨酸测定法测定的心肌胶原含量呈线性相关(r=0.78)。定量组织学分析显示，胶原蛋白的透壁分布与相应的背向散射信号的透壁模式之间存在着不同的关系。在2例表现为心内膜下纤维化层的标本中，心内膜下区域的背向散射幅度也增加。在其他纤维化类型的标本中，局部背向散射的幅度与胶原分布的跨壁模式不一致。超声背向散射定量分析为无创评价心肌梗死后心肌纤维化提供了可能。
In animal hearts, the magnitude of integrated ultrasonic backscatter is increased in fibrotic myocardium. The relationship between ultrasonic backscatter and collagen deposition was quantitated in 10 excised human hearts with old infarcts. A 2.25 MHz, 50% fractional bandwidth transducer was positioned at the transducer focal distance from the epicardium of each specimen. The radio frequency backscatter signal was digitized, squared and integrated to yield the integrated ultrasonic backscatter, which was referenced to the backscatter from a water/steel interface. The interrogated myocardium was then excised and divided into 2 portions. One portion was assayed for hydroxyproline, a marker for collagen. A 2nd portion was sectioned, stained with Masson''s trichrome, and studied with the use of a computer-assisted image analysis system. There was a linear correlation between the magnitude of integrated backscatter and myocardial collagen content estimated by hydroxyproline assay (r = 0.78). Quantitative histologic analysis revealed a variable relationship between the transmural distribution of collagen and the corresponding transmural pattern of the backscatter signal. In 2 specimens exhibiting a discrete layer of subendocardial fibrosis, the backscatter amplitude was also increased in the subendocardial region. In specimens with other patterns of fibrosis, the local backscatter amplitude did not correspond to the transmural pattern of collagen distribution. The quantitative analysis of ultrasonic backscatter shows promise for the noninvasive evaluation of myocardial fibrosis after infarction.